| 摘要: |
| 利用MTT法检测细叶卷柏乙酸乙酯和正丁醇提取物对HeLa细胞生长的抑制作用,利用流式细胞术(FCM)比较不同提取物对细胞凋亡的影响。结果显示:细叶卷柏的乙酸乙酯和正丁醇部位抑制细胞生长和诱导细胞凋亡作用均有明显的剂量依赖性。乙酸乙酯部位的IC50值为1.927 μg/mL,正丁醇部位的IC50值为24.600 μg/mL。因此,细叶卷柏乙酸乙酯部位的体外抗肿瘤活性相对较强,其次是其正丁醇部位,水提部位相对较弱。细叶卷柏是一种潜在的抗肿瘤药用植物。 |
| 关键词: 细叶卷柏 抗肿瘤 MTT FCM |
| DOI： |
| 分类号:Q946 |
| Fund project: |
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| Anti-tumor activities in vitro of extracts from Selaginella labordei |
|
LI Juan1,2, CHEN Ke-Li2*, XU Jia-Cheng2
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1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006,
China;2.Hubei College of Traditional Chinese Medicine, Key Laboratory of TCM Resource and TCM Compound
Co-constructed by Hubei Province and Ministry of Education, Wuhan 430065, China
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| Abstract: |
| MTT assays were used to evaluate effects of ethyl acetate and n-butanol extracts from Selaginella labordei on inhibiting cell proliferation. Flow cytometry(FCM)was used to detect cell apoptosis rate. The results showed that ethyl acetate and n-butanol extracts had a distinctive dose-dependent relation in inhibiting cell proliferation and inducing cell apoptosis. The IC50 values of ethyl acetate and n-butanol extracts were 1.927 μg/mL and 24.600 μg/mL respectively. So ethyl acetate extracts possessed stronger anti-tumor activity,and n-butanol extracts the next,water extracts were relatively weak. S.laborei is a potential medical plant with anti-tumor activities. |
| Key words: S.laborei anti-tumor activities MTT FCM |
