罗汉果不同溶剂提取物抗氧化及清除活性氧自由基作用

Antioxidant and activated oxygen free radical scavenging activities of different extract from Siraitia grosvenorii

关键词:  罗汉果  总抗氧化性  超氧阴离子自由基  羟基自由基  脂质过氧化

Key words:  Siraitia grosvenorii  total antioxidant capacity  superoxide anion radical  hydroxyl radical  lipid peroxidation

摘要:

以水、甲醇、乙醇和乙酸乙酯为溶剂,对罗汉果干果进行提取,分别采用磷钼酸铵体系、邻苯三酚自氧化体系、Fenton反应体系和卵黄脂质过氧化体系测定各种提取物的总抗氧化性能、超氧阴离子自由基和羟基自由基清除性能及其抗脂质过氧化作用。结果表明,四种溶剂提取物均具较强的抗氧化性和活性氧自由基清除性能,其能力的大小顺序为:乙酸乙酯提取物>水提物>甲醇提取物>乙醇提取物。

Abstract:

The antioxidant and radical scavenging activities of water extract,methanol extract,ethanol extract and ethyl acetate extract from dried fruit of Siraitia grosvenorii were investigated in present study,employing various methods established in vitro systems,such as the total antioxidant capacity measured by phosphombdenum method,scavenging activities towards superoxide anion radical with auto-oxidation of pyrogallol method,scavenging activities towards hydroxyl radical with fenton reaction,and antilipoperoxidation activities measured by method of peroxidation of egg lipid. The results showed that all the extracts exhibited antioxidant and radical scavenging activities at different magnitudes of potency. The decreasing order of antioxidant and radical scavenging activities among the extracts assayed through all the four methods were found to be ethyl acetate extract>water extract>methanol extract>ethanol extract. Results of this study show that extracts of S.grosvenorii might be valuable antioxidant natural sources and seem to be applicable in both healthy medicine and foods.