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| 实时荧光定量PCR检测三七SS基因表达的初步实践 |
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朱 华1, 吴耀生2
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1.桂林医学院 生物化学与分子生物学教研室,广西 桂林 541004;2.广西
医科大学 生物化学与分子生物学教研室, 南宁 530021
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| 摘要: |
| 运用含有SYBR Green I的Real Time RT-PCR法分析SS基因在一年生三七根、茎、芦头3个部位中转录水平的相对表达差异。统计分析表明SS基因在根中的表达量最高。本研究取得了特异性高、重复性好的结果,标准曲线斜率均在-3.33～-4范围内,扩增效率均在95%～100%之间,熔解曲线分析显示产物特异性的单一峰,为Real Time RT-PCR技术用于三七植物基因的差异表达分析建立了相应的技术平台。 |
| 关键词: Real Time RT-PCR 三七 SS基因 表达差异 |
| DOI： |
| 分类号:Q946 |
| Fund project: |
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| Initial practice of Real Time PCR for the expression of SS gene in Panax notoginseng |
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ZHU Hua1, WU Yao-Sheng2
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1.Department of Biochemistry, Guilin Medical College, Guilin 541004, China;2.Department of
Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| The total RNA was isolated from root,stem and rootstock of one-year-old Panax notoginseng respectively. Then, the transcripts of SS gene in the three tissues were assayed by SYBR Green I Real Time RT-PCR. The results revealed that SS gene is highest expressed in root. In this research, the results showed high specificity and stability with the standard curve slope between -3.33 and -4; the PCR efficiency between 95%-100%; and the exclusive peak in melting curve. All these would make the technique goes smoothly in the analyzing of the differential expression in P.notoginseng. |
| Key words: Real Time RT-PCR Panax notoginseng SS gene differential expression |
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