蔓花生组织培养和植株再生的条件优化

Condition optimization for tissue culture of Arachis duranensis

关键词:  蔓花生  组织培养  愈伤组织

Key words:  Arachis duranensis  tissue culture  callus

摘要:

应用MS为基本培养基,通过各种培养条件和不同激素配比,探讨蔓花生组织培养及其植株再生条件的优化。结果显示:幼叶为最佳的外植体,在幼叶愈伤诱导过程中,不超过12 h光照,光照强度在21.6 μmol·m^-2·s^-1均可; 诱导愈伤组织的适宜培养基为MS+0.5 mg/L6-BA+0.2 mg/L2,4-D或MS+0.5 mg/L6-BA+2 mg/LNAA; 最适的分化培养基为MS+1 mg/LTDZ+2 mg/L6-BA+0.5 mg/LNAA; 最适的生根培养基1/2MS+1 mg/LNAA+1 mg/LPP₃₃₃。

Abstract:

Condition optimization for tissue culture and regeneration of Arachis duranensis was studied. The results showed that the leaflet was the best experimental explant; the optimized medium for callus was MS+0.5 mg/L6-BA+0.2 mg/L2,4-D or MS+0.5 mg/L6-BA+0.2 mg/LNAA with illumination of 12h/d; the optimized medium for differentiation was MS+1 mg/LTDZ+2 mg/L6-BA+0.5 mg/LNAA; the optimized medium for root induction was 1/2MS+1 mg/LNAA+1 mg/LPP₃₃₃.