| 摘要: |
| 以匙羹藤无菌种子苗为外植体,通过预备实验确定基本培养基后,采用完全实验和正交设计相结合的方法,研究植物生长调节剂两因素配合和多因素组合(NAA、TDZ、2-ip、IAA、6-BA和KT)对匙羹藤芽体诱导及继代培养的影响。结果显示,匙羹藤组织培养最适的基本培养基为N68; N68+NAA 0.05 mg/L+2-ip0.4 mg/L+TDZ 0.001 mg/L利于芽体增殖,可用于继代培养; N68+NAA 0.05 mg/L+TDZ 0.005 mg/L促进植株长根,可用于生根培养。 |
| 关键词: 匙羹藤 组织培养 完全实验 正交设计 |
| DOI： |
| 分类号:Q943.1 |
| Fund project: |
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| Optimization in vitro culture conditions for Gymnema sylvestre |
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SU Tai1,2, HUANG Ning-Zhen1*, FU Chuan-Ming1
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1. 1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences,
Guilin 541006, China;2.College of Life Sciences, Guangxi Normal University, Guilin 541004, China
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| Abstract: |
| The optimum medium for tissue culture of Gymnema sylvestre were studied by using germfree tub-seedling as explants. The effects of plant growth regulator(NAA,TDZ,2-ip,IAA,6-BA and KT)on the seedlings proliferation were studied by complete experiment and orthogonal design. The results showed that the optimum basic medium for G.sylvestre was N68; N68+NAA 0.05 mg/L+2-ip 0.4 mg/L+TDZ 0.001 mg/L was the best for bud proliferation,and can be used in subculture; N68+NAA 0.05 mg/L+TDZ 0.005 mg/L promoted rooting,can be used as rooting medium. |
| Key words: Gymnema sylvestre tissue culture complete experiment orthogonal design |
