西北黄芪和山野豌豆对低温胁迫的生理响应研究

Physiological response of Astragalus fenzelianus and Vicia amoena to low temperature stress

关键词:  豆科牧草  低温胁迫  生理指标  

Key words:  legume  low temperature stress  physiological characteristic

摘要:

以两种野生豆科牧草(西北黄芪和山野豌豆)为试验材料,研究其对低温胁迫的生理响应。结果表明:在低温胁迫下,西北黄芪和山野豌豆的相对电导率、SOD活性、可溶性糖含量、脯氨酸含量均表现出上升的趋势,且随着低温胁迫程度的加剧而加深; 就相对电导率、可溶性糖含量两项生理指标而言,西北黄芪5 ℃增幅显著小于山野豌豆,而-5 ℃对5 ℃增幅显著大于山野豌豆; 就脯氨酸含量而言,西北黄芪5 ℃增幅大于山野豌豆,而-5 ℃对5 ℃增幅显著小于山野豌豆; 就SOD活性而言,西北黄芪5 ℃增幅大于山野豌豆,而-5 ℃对5 ℃增幅显著大于山野豌豆。综合分析,认为西北黄芪比山野豌豆具更强的抗低温能力,同时说明,两种牧草对低温胁迫的生理响应规律不同,相对电导率和可溶性糖含量两项生理指标对低温的响应更加敏感。

Abstract:

Effects of low temperature stress on physiological characteristics of A.fenzelianus seedlings and V.amoena seedlings were studied. The results showed that the conductance rate,the soluble sugar content,the proline content and the SOD activity were increased. Taking the conductance rate and the content of soluble sugar as index,the increased degree in A.fenzelianus seedlings was significantly lower than that of V.amoena seedlings at 5 ℃ and significantly higher than that of V.amoena seedlings in -5 ℃ contracted with the 5 ℃; taking the proline content as index,the increased degree in A.fenzelianus seedlings is significantly higher than that of V.amoena seedlings at 5 ℃ and significantly lower than that of V.amoena seedlings in -5 ℃ contracted with the 5 ℃; taking the SOD activity as index,the increased degree of A.fenzelianus seedlings was significantly higher than that of V.amoena seedlings at 5 ℃ and in 5 ℃ contracted with the -5 ℃. Integrating the physiological characteristic indices,it was considered thatA.fenzelianus seedlings showed stronger cold resistance ability to low temperature than V.amoena seedlings. It's therefore concluded that these two kinds of seedings responds differently to low temperature in terms of physiological characteristics and the conductance rate,and the content of soluble sugar is more sensitive to low temperature.