重组水母发光蛋白及其在植物钙离子信号检测中的应用

Recombinant aequorin luminous protein and its application in measuring calcium signal of plant cell

关键词:  重组水母发光蛋白  钙浓度换算  〖Ca²⁺〗_cyt  钙信号

Key words:  recombinant aequorin luminous protein  conversion to the calcium concentration  〖Ca²⁺〗_cyt  calcium signal

摘要:

重组水母发光蛋白作为检测植物细胞钙信号的手段是近十几年发展起来的新方法,该文介绍了重组水母发光蛋白作为Ca²⁺检测探针的发展过程、测钙原理、Ca²⁺浓度检测方法、Ca²⁺浓度换算方法、优点与不足、及在植物细胞钙离子信号检测中的研究进展。并利用国外实验室提供的方法在国内首次得出冷激条件下植物细胞内细胞质中(〖Ca²⁺〗_cyt)和液泡膜附近(〖Ca²⁺〗_md)钙离子浓度动力学变化曲线。

Abstract:

Recently,the application of recombinant aequorin luminous protein has appeared in measuring calcium signal of plant cell as a new method. This paper introduced mainly the development of the recombinant aequorin luminous protein as a probe,the principle and operates method of measuring calcium concentration,and how to conversion the calcium concentration. It summarized the advantages and deficiencies of the protein,and collected mainly the progress in development studies in measuring calcium signal of plant cell. Meanwhile,using the method provided by other laboratory,the authors got the cold shock kinetics of 〖Ca²⁺〗_cyt and 〖Ca²⁺〗_md for the first time.