亚洲百合花丝离体培养器官形成的细胞形态学研究

Cytomorphology of organogenesis in filament

关键词:  亚洲百合  花丝  器官形成  细胞形态学

Key words:  Lilium Asiatic Hybrids  filament  organogenesis  cytomorphology

摘要:

对亚洲百合的花丝进行离体培养,并利用常规石蜡制片技术对诱导效果最好的材料进行细胞形态学观察,研究花丝在离体培养过程中器官形成的细胞形态学变化。结果表明:花丝在MS+BA0.5 mg/L+NAA0.5 mg/L的培养基上诱导效果最好。离体培养后其形态学下端切口内方的1～3层细胞首先启动脱分化,然后是内方的10～12层细胞,而其他部位的细胞自始至终未启动脱分化。亚洲百合的再生方式为器官发生型,器官通过胚性愈伤组织间接产生,在胚性愈伤组织团表面附近形成芽原基,或在胚性愈伤组织团内部形成根原基,有时同时分别在内、外形成根原基和芽原基后再通过维管组织连接成完整的植株。本研究为亚洲百合的人工调控提供基础理论依据。

Abstract:

Filaments of Lilium Asiatic Hybrids were explanted on MS media supplemented with different combinations of BA and NAA to induce organogenesis and somatic embryogenesis,and the cytohistological changes of explants were studied using paraffin section technology. The main results were as follows: the best medium was MS+BA0.5 mg/L+NAA0.5 mg/L. Callus was initiated from 1-3 layers of cells on the cut surface of the morphological lower side of explants,and then 10-12 layers of cells returned to division; but cells from other positions were not initiated. The adventitious shoots and roots which originated from the embryogenic callus packs were formed by organogenesis on the surface or from inner cells. Vascular tissue was finally formed to connect adventitious shoots and roots. The research would provide the theoretical basis for artificial control on Lilium Asiatic Hybrids.