苦豆子的组织培养及植株再生的研究

Tissue culture and plant regeneration of Sophora alopecuroides

关键词:  苦豆子  组织培养  植株再生

Key words:  Sophora alopecuroides  tissue culture  plant regeneration

摘要:

用4种诱导培养基P1(MS+2,4-D 0.5 mg/L)、P2(MS+6-BA 0.1 mg/L+NAA 0.5 mg/L)、P3(MS+6-BA 0.5 mg/L+NAA 0.5 mg/L)、P4(MS+NAA 1.0 mg/L+KT 0.5 mg/L),3种分化培养基(MS+6-BA 1 mg/L; MS+6-BA 0.5 mg/L; MS+6-BA 1 mg/L+NAA 0.2 mg/L)和4种生根培养基(MS; MS+IBA 1 mg/L; MS+IBA 1 mg/L+IAA 0.5 mg/L; 1/2 MS+IAA 0.2 mg/L)对苦豆子愈伤组织进行诱导和植株再生,研究影响苦豆子组织培养的因素,结果表明愈伤组织的诱导频率主要依靠激素的种类和浓度,培养基中加入0.2～2 mg/L的2,4-D有利于苦豆子愈伤组织生长,但使褐化发生时间提前; 培养基中加入活性炭对苦豆子愈伤组织褐化有明显的抑制作用; 加入IAA对苦豆子根的分化是必需的。

Abstract:

In order to study the factors that influence the tissue culture of Sophora alopecuroikes,four callus-inducing media,i.e.P1(MS+2,4-D 0.5 mg/L),P2(MS+6-BA 0.1 mg/L+NAA 0.5 mg/L),P3(MS+6-BA 0.5 mg/L+NAA 0.5 mg/L),P4(MS+NAA 1.0 mg/L+KT 0.5 mg/L),three different media(MS+6-BA 1 mg/L; MS+6-BA 0.5 mg/L; MS+6-BA 1 mg/L+NAA 0.2 mg/L),and four root-inducing media(MS; MS+IBA 1 mg/L; MS+IBA 1 mg/L+IAA 0.5 mg/L; 1/2 MS+IAA 0.2 mg/L)were applied. The results showed that the induction frequency of calluses highly depended on the varieties and concentration of hormone in media. 2,4-D in media,with concentration ranging from 0.2-2 mg/L,was favorable to the growth of calluses of S.alopecuroikes,accompanying earlier browning occurrence. It was found that adding active charcoal into medium could efficiently inhibit the callus browning. In addition,IAA seemed an essential ingredient in medium to the root differentiation of S.alopecuroikes.