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| 利用SSR标记与毛细管电泳对甘蔗属进行的遗传分析 |
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梁 俊1,2,3,4, PAN Yong-Bao6, 李杨瑞1,2,4,5*, 方锋学2,3,4
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1.广西大学 广西亚热带生物资源保护利用重点实验室, 南宁 530004;2.中国农业科学院 甘蔗研究中心, 南宁
530007;3.农业部 甘蔗品质监督检验测试中心, 南宁 530007;4.国家糖料改良中心 广西甘蔗品种改良分
中心, 南宁 530007;5.广西作物遗传改良生物技术重点开放实验室, 南宁 530007;6.6.USDA-ARS,
Sugarcane Research Unit, 5883 USDA Road, Houma, LA 70360, U.S.A.
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| 摘要: |
| 为探讨甘蔗属内不同种之间的遗传多样性,利用SSR标记与毛细管电泳技术,对来自甘蔗属3个不同种的12个材料19对引物进行检测,共检测到229个DNA多态性条带,19对引物扩增的DNA条带范围集中在100～260 bp之间。12个甘蔗材料的Jaccard遗传相似度,最小0.09,最大0.65,平均为0.26。通过遗传相似性系数分析,UPGMA聚类图内12个甘蔗材料可分为两个群,三个割手密种材料分为一个亚群,甘蔗栽培品种与甘蔗热带种合为一个亚群。结果表明:热带种比割手密种具有和甘蔗栽培品种更亲近的遗传关系; SSR分子标记与毛细管技术结合,相比别的分子标记技术或电泳技术,具有更准确、简便、自动化等优点。 |
| 关键词: 简单重复序列(SSR) 甘蔗 毛细管电泳 |
| DOI： |
| 分类号:Q943 |
| Fund project: |
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| The work was conducted at the USDA-ARS,Sugarcane Research Laboratory,Houma,LA,U.S.A. under a Non-funded Cooperative Agreement(USDA Control No. 410334)between the Sugarcane Research Center,Chinese Academy of Agricultural Sciences,Nanning 530007 and the USDA- ARS,Sugarcane Research Laboratory,Houma,LA 70360,U.S.AAssessment of genetic diversity in Saccharum using SSR markers and capillary electrophoresis |
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LIANG Jun1,2,3,4, PAN Yong-Bao6, LI Yang-Rui1,2,4,5*, FANG Feng-Xue2,3,4
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1.Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530004, China;2.Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China;3.Sugarcane Quality Inspection
and Test Center at Nanning, Ministry of Agriculture, Nanning 530004, China;4.Guangxi Sugarcane Variety Branch, National
Sugar Crops Improvement Center, Nanning 530007, China;5.Guangxi Crop Genetic Improvement and Biotechnology Lab,
Nanning 530007, China;6.6.USDA-ARS, Sugarcane Research Unit, 5883 USDA Road, Houma, LA 70360,U.S.A.
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| Abstract: |
| This study was conducted to assess the genetic diversity amongst 12 Saccharum clones from 3 species using 19 SSR markers and capillary electrophoresis(CE). A total of 229 DNA bands were generated showing a size range between 100 and 260 bp. Based on the SSR data,the Jaccard's genetic similarity coefficients ranged from a minimum of 0.09 between CP72-1210(cultivar)and In81-142(S.spontaneum)to a maximum 0.65 between GX87(S.spontaneum)to GX86(S.spontaneum). In the dendrogram using UPGMA method,the 12 Saccharum clones were clustered into two groups. The three S.spontaneum clones formed a single group,while the three S.officinarum clones clustered with the six cultivars,which demonstrated that S.officinarum had a closer genetic relationship with cultivars than S.spontaneum. The SSR markers data generated based on capillary electrophoresis are more accurate,simpler and automatic as compared to other molecular markers or electrophoresis. |
| Key words: Simple Sequence Repeats(SSR) sugarcane capillary electrophoresis |
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