| 摘要: |
| 以采自云南丽江的松茸子实体为材料,进行组织分离后,利用一对ITS引物（ITS1 ITS4）对子实体（SR176B,SR172B）和分离物（SR176H,SR172H）进行了PCR扩增、琼脂糖凝胶电泳分析,得到了700 bp左右的扩增条带,进一步对ITS序列进行同源性检索比对,结果表明SR176H与SR176B,SR172H与SR172B序列同源性均为100%,鉴定出该分离物就是松茸的纯培养物。 |
| 关键词: 松茸 子实体 分离物 ITS序列 |
| DOI： |
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| Fund project: |
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| Identification of cultures isolated from fruit body of Tricholoma matsutake by rDNA-ITS sequence analysis |
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ZHANGLing1,2,ZHAOQi1,CHENYan-Ping1,MENGZhen-Gui1,LIRong-Chun1*
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1.Insititution of Edible Fungi, Yunnan Agricultural University, Kunming 650201, China;2.College of Science and Information Engineering, Yunnan Agricultural University, Kunming 650201, China
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| Abstract: |
| A pair of general primer(ITS1 ITS4)was used to test in the amplification of the internal transcribed spacer(ITS)region of the chromosomal DNA of fruit body of Tricholoma matsutake of Lijiang and its isolates. The results analyzed by Agarose gel electrophoresis showed that the primers of ITS1 and ITS4 amplified about 700bp fragments for all samples. The ITS fragments of T.matsutake fruit body and its corresponding isolates were sequenced and aligned.Their sequences of SR176H and SR176B showed a 100% homogeneity,the same result also appeared between SR172H and SR172B.Based on these facts,SR176H and SR172H were confirmed to be pure cultures of T.matsutake. |
| Key words: Tricholoma matsutake fruit body mycelia rDNA-ITS |
