黄瓜无菌苗雌花诱导体系的建立

Establishment for induction system of female flowers in steriled seedlings of Cucumis sativus

关键词:  黄瓜  无菌苗  雌花  诱导体系

Key words:  Cucumis sativus  steriled seedlings  female flowers  induction system

摘要:

研究Spm和IAA对无菌黄瓜苗雌花诱导的协同作用,及不同外植体、培养基中琼脂含量和KH2PO4含量对雌花诱导的影响,由此建立了有效的雌花诱导体系。黄瓜去根苗接种在MS培养基上,单独添加Spm、IAA时的雌花诱导率、雌花数偏低或为0,同时添加12 mg•L-1 Spm与0.01 mg•L-1 IAA时达21%、29枚,对照组未见雌花,说明Spm和IAA对雌花诱导的协同作用显著。实验证明,在全苗、去根苗、去根去顶苗、顶芽四种外植体中,及在0.5%～0.9%琼脂含量和1.0～2.0 mmol•L-1 KH2PO4含量的培养条件下,采用07%琼脂含量和1.75 mmol•L-1KH2PO4含量培养黄瓜去根去顶苗的雌花诱导效果最好,其诱导率和雌花数分别达46%、54枚。

Abstract:

The synergistic effect of Spm and IAA,and the effects of agar concentration,KH2PO4 concentration on the induction of female flowers in steriled seedlings of Cucumis sativus for the establishment of an induction system of female flowers were reported. It was found that the induction rate and the amount of female flower in every trial were considerably low and even down to zero when Spm or IAA was respectively added to derooted seedlings cultured on MS basal medium,while the induction effect were obviously higher when 12 mg•L-1 Spm and 0.01 mg•L-1 IAA were added simultaneously,and no female flowers could be observed on the control medium,which showed that Spm and IAA had an significant synergistic effect on the induction of female flowers. Experiment was done with different explant of whole seedlings,derooted seedlings,detoped seedlings without root,top shoots or with agar concentration of 0.5%-0.9%,and with KH2PO4 concentration of 1.0-2.0 mmol•L-1,the highest induction effect for female flowers was achieved on medium of agar 0.7%,KH-2PO-4 1.75mmol•L-1 and from detoped seedlings without root,its induction rate and amount of female flower reched to 46%,54 respectively.