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| 应用胚挽救技术获得三倍体柑橘植株 |
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刘可慧1,2,于方明3,张秋明4,易干军1*
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1.广东省农业科学院 果树研究所, 广州 510640;2.桂林电子科技大学 应用科技学院, 广西 桂林 541004;3. 3.广西师范大学 环境与资源学院, 广西 桂林 541004;4.湖南农业大学 园艺园林学院, 长沙 410128
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| 摘要: |
| 以3个柑橘异源四倍体体细胞杂种(即四倍体粗柠檬与哈姆林甜橙体细胞杂种,简称“HR”;酸柚与粗柠檬体细胞杂种,简称“SR”;墨西哥来檬与伏令夏甜橙体细胞杂种,简称“KV”)为父本,分别与二倍体单胚性沙田柚进行有性杂交,在有性胚还没有完全败育以前,通过胚抢救技术进行三倍体植株培养（以四季柚花粉亲本为对照）。结果表明,处理的杂交组合直接成苗率极显著低于对照(P＜0.01),其中又以沙田柚×HR组合成苗率相对较高,达10.1%;较适合瘪籽沙田柚幼胚离体培养的培养基是MT+GA31.0 mg/L+蜂皇浆200 mg/L+水解乳蛋白250 mg/L+蔗糖30 g/L+琼脂0.7%;流式细胞分析仪检测结果显示,本试验成功获得了柑橘三倍体植株。 |
| 关键词: 体细胞杂种 有性杂交 瘪籽沙田柚 胚抢救 三倍体植株 |
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| Regenerating triploid citrus plants by embryo rescue technique |
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LIUKe-Hui1,2,YUFang-Ming3,ZHANGQiu-Ming4,YIGan-Jun1*
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1.Institute of Pomology, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;2.College of Applied Science and Technology, Guilin University of Electronic Technology, Guilin 541004, China;3.3.Department of Environmental Engineering, Guangxi Normal University, Guilin 541004, China;4.4.College of Horticulture, Hunan Agricultural University, Changsha 410128, China
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| Abstract: |
| Diploid Shatian pummelo was used as the female and crossed with the allotetraploid somatic hybrids(Citrus jambhiri+C.sinensis(HR);C.grandis+Rough lemon(SR);C.aurantifolia+C.sinensis(KV))to regenerate triploid citrus plants by embryo rescue technique,using C. grandis pollen as the control. Results showed that the regenerating rate of treated sexual hybridization was significantly lower than that of the control(P＜0.01),in which the corresponding value of Shatian pummelo×HR was comparably higher(the rate of seedling is 101%). The optimal medium for embryo rescue was solid MT medium supplemented with GA3 1.0 mg/L,queen bee oar 200 mg/L,lacialbumin hydrolysate 250 mg/L and agar 0.7%. Results of flow cytometry analysis showed that triploid citrus plants were successfully obtained in this study. |
| Key words: somatic hybrids hybridizing seedless Shatian pummelo embryo rescue triploid plants |
