盐胁迫对大米草幼苗某些生理指标的影响

Effects of salt stress on some physiological characteristics of Spartina anglica seedling

关键词:  大米草  盐胁迫  保护酶  生理指标

Key words:  Spartina anglica  salt stress  cell defense enzymes  physiological indicator

摘要:

研究了大米草幼苗在不同培养盐度（0、20、30、50、100 mmol/L NaCl）下,MDA、游离脯氨酸、可溶性糖、可溶性蛋白质含量以及保护酶（SOD、POD、CAT）活性等生理指标的变化情况。结果表明：保护酶（SOD、POD、CAT）活性在盐胁迫40d前逐渐上升且达显著差异。随着胁迫时间延长,MDA含量与CK相比逐渐降低。随着盐分胁迫浓度的增加及盐胁迫时间延长,大米草叶片中游离脯氨酸、可溶性糖、可溶性蛋白质含量呈上升趋势。在盐胁迫下,渗透调节物质的积累作用是大米草对盐胁迫的主要响应过程,其体内的抗氧化保护酶在此过程中也发挥了重要的作用。

Abstract:

Under different salinity conditions（0,20,30,50,100 mmol/L NaCl）,variations of some physiological indicators including MDA,free praline,soluble sugar,soluble protein contents and antioxidative enzymes(SOD,POD,CAT)activity of Spartina anglica seedlings were investigated. The results were showed as follows:the antioxidative enzymes(SOD,POD,CAT)activity was gradually increasing during the forty days under salt stress and there were significant differences with the prolongation of time salt stress. Compared with ckeck,the MDA contents generally decreased with the prolongation of time salt stress. The free proline,soluble sugar and soluble protein contents in leaves showed an increasing trend with increasing salinity and the prolongation of time salt stress. Accumulation of osmoprotectants was the key response of S.anglica salinity stress,and the antioxidative enzymes played important roles in it.