福建地区小叶买麻藤遗传多样性ISSR分析

Genetic diversity of Gnetum parvifolium of Fujian by ISSR Markers

关键词:  小叶买麻藤  遗传多样性  遗传结构  种群退化

Key words:  Gnetum parvifolium  genetic diversity  genetic structure  population degradation

摘要:

采用13条ISSR引物对福建地区小叶买麻藤11个种群共211个样本进行了种群遗传多样性检测。结果表明：(1)小叶买麻藤在物种水平上遗传多样性较高而在种群水平上较低，揭示该物种具有较强的生存、适应、发展潜力，但其种群遗传多样性已经受到生境片段化及人为活动的影响；(2)小叶买麻藤的遗传分化在裸子植物中处于中等水平，选择和基因流对种群遗传分化的作用大于遗传漂变的作用；(3)小叶买麻藤种群退化主要受人类活动影响，影响的时间较短，尚未表现出种群遗传结构的改变。

Abstract:

Thirteen ISSR primers were selected to reveal the population genetic diversity of Gnetum parvifolium in Fujian. 211 samples across 11 populations were examined. The results showed that:(1)G.parvifolium exhibited comparatively high genetic diversity at species level while comparatively low at population level，indicating that this species had a strong potential for survival，adaptation and expansion，but its population genetic diversity had been effected by habitat fragmentation and human activity；(2)the genetic differentiation of G.parvifolium was among the moderate level of gymnosperm，which was mainly caused by selection pressure and gene flow instead of genetic drift；(3)human activities contributed to the population degradation of G.parvifolium，but this degradation had little detectable effect on the genetic structure of the target species due to limited impactive time.