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缺铁和过量重碳酸盐胁迫下丛枝菌根真菌对枳活性氧代谢的影响
王明元1, 夏仁学2
1.华侨大学 化工学院生物工程与技术系, 福建 厦门 361021;2.华中农业大学 园艺植物生物学教育部重点实验室, 武汉 430070
摘要:
采用盆栽沙培试验,研究了缺铁处理及重碳酸盐胁迫下丛枝菌根真菌地表球囊霉对枳实生苗抗活性氧系统的影响。试验结果表明,在缺铁以及重碳酸盐胁迫处理下,丛枝菌根真菌显著提高了枳叶片和根系中可溶性蛋白含量、超氧化物岐化酶活性、过氧化物酶活性和过氧化氢酶活性,明显增强了叶片类胡萝卜素含量,显著降低了枳叶片和根系中的丙二醛含量,增强了枳自身防御能力,减少了胁迫对枳细胞膜的伤害。
关键词:  丛枝菌根真菌  活性氧代谢  枳实生苗  重碳酸盐
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Effects of Glomus versiforme on active oxygen metabolism of Poncirus trifoliate under iron deficiency and heavy bicarbonate stress
WANG MingYuan1, XIA RenXue2
1.Department of Bioengineering and Biotechnology, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China;2.Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
Abstract:
Effects of protective or defensive system on trifoliate inoculated arbuscular mycorrhizal fungus,Glomus versiforme,were investigated on sand culture under iron deficiency and bicarbonate stress. The results showed that the soluble protein contents and the SOD,POD and CAT activities of root or leaf were significantly higher in mycorrhizal plants than that in non mycorrhizal plants under iron deficiency and bicarbonate stress. Additionally,carotinoid contents in leaves were enhanced and malindialdehyde contents in both leaves and roots were significantly decreased,which suggested that the arbuscular mycorrhizal fungus G.versiforme was able to enhance the defensive ability of plant itself to avoid harm on cells of trifoliate.
Key words:  arbuscular mycorrhizal fungus  active oxygen metabolism  trifoliate orange(Poncirus trifoliata)  bicarbonate
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