SNP和cPTIO对NaCl胁迫下拟南芥的生理影响

Effects of SNP and cPTIO on physiology of Arabidopsis seedling under NaCl stress

关键词:  一氧化氮  cPTIO  盐胁迫  拟南芥

Key words:  nitric oxide(NO)  cPTIO  NaCl stress  Arabidopsis thaliana

摘要:

以拟南芥为材料，研究了0.5 mmol/L的外源一氧化氮供体硝普纳（SNP）和200 mmol/L的一氧化氮专一清除剂（cPTIO）对200 mmol/L NaCl胁迫下对拟南芥的生理影响。0.5 mmol/L SNP和200 mmol/L cPTIO预处理2 h后，加入200 mmol/L NaCl。不同处理的拟南芥加入200 mmol/L NaCl后，每隔12 h取样一次，观察叶片生长情况、测定过氧化物酶(POD)、超氧化物歧化酶(SOD)、丙二醛（MDA）和超氧阴离子（O2）的变化。结果表明0.5 mmol/L的SNP能缓解200 mmol/L NaCl胁迫伤害，促进盐胁迫下幼苗的生长，显著提高抗氧化酶系统中POD和SOD活性，显著降低MDA和O2的含量，从而提高植株的抗盐性，而NO专一清除剂cPTIO能逆转SNP的上述效应。

Abstract:

Three groups of 8 week old Arabidopsis seedlings were cultured，they were respectively pretreated with water，0.5 mmol/L SNP and 200 mmol/L cPTIO for 2 h，after that，200 mmol/L NaCl was added. Fresh NaCl solution was applied every day and some physiological indices such as peroxidase(POD)，superoxide dismutase(SOD)，malondialdehyde(MDA)and superoxide anion(O2)were determined 12 h later. The results showed that SNP could relieve NaCl stress injuries，and promote the growth of seedlings under salt stress，significantly improve the antioxidant enzyme system，POD and SOD activity，significantly reduce MDA and O2 content，thereby enhancing the salt tolerance of plants，while the NO specific scavenger cPTIO could reverse the above effects of SNP.