胶束电动毛细管色谱法测定红丝线提取物中紫蓝素的含量

Determination of zilanine contents in extracts of Peristrophe baphica by Micellar Eletrokinetic Capillary Chromatography (MECC)

关键词:  胶束电动毛细管电泳色谱法  紫蓝素  红丝线提取物

Key words:  MECC  Zilanine  extract of Peristrophe baphica

摘要:

建立了胶束电动毛细管电泳色谱法(MECC)测定红丝线提取物中紫蓝素的含量的方法。毛细管柱内径75 μm，长50.2 cm；运行电压25 kV，检测波长585 nm，温度25 ℃；缓冲液为25 mmol/L，β CD 10 mmol/L，硼酸盐 20%乙腈（pH值8.0）；进样方式：压力进样，进样时间5 s。结果表明紫蓝素在10～100 μmol/L浓度范围内具有良好的线性关系(r=0.9995)，回收率为95.3%～103.2%。该方法快速、简便、并且较为准确，适用于测定红丝线提取物中紫蓝素的含量。

Abstract:

The method of MECC was established to determine zilanine contents in extracts of P.baphica. The capillary column was 50.2 cm long and 75 μm in internal diameter；the operation voltage was 25 kV，testwavelength 585 nm，column temperature 25 ℃；the mobile buffer was 25 mmol/L boric acid 20% acetonitrile(pH 8.0)with 10 mmol/L β CD；the sampling was conducted by pressure for 5 s. The results showed that the linear range of determination for zilanine was 10～100 μmol/L(r=0.9995). The average recovery rate was 953%-103.2%. The method was fast，simple，convenient，comparatively accurate，and could be used to determinate zilanine contents in extract of P.baphica.