应用蚕豆根尖细胞微核技术评价广西平乐锰矿废弃地土壤的污染状况

Evaluation on soil pollution in Pingle Manganese Mine of Guangxi using Vicia faba root-tip cells micronucleus testing

关键词:  蚕豆根尖细胞微核技术  锰矿废弃地  土壤污染  污染指数  广西

Key words:  Vicia faba  micronucleus technique  manganese mine wasteland  soil pollution  Guangxi

摘要:

分别选取废弃地上的花生地、柿树地、柑桔地和桃树地等4个采样点进行调查和监测,测定了各采样点土壤浸提物的蚕豆根尖细胞微核千分率(MCN‰)和污染指数(PI),应用蚕豆根尖细胞微核技术对广西平乐锰矿废弃地土壤的污染状况进行了评价,并对造成土壤污染的原因进行了分析讨论。结果表明,用浸提法处理的4个采样点土壤的根尖细胞微核千分率(MCN‰)均明显高于对照组(P<0.05),其中柑桔地的污染程度最严重,污染指数达到了2.93; 污染程度最轻的是花生地,其污染指数为1.97。这表明广西平乐锰矿废弃地土壤均存在不同程度的污染,在锰矿废弃地土壤上早期不宜种植食用的农作物。

Abstract:

In order to study the pollution status of soil in Pingle Manganese Mine wasteland of Guangxi,four sampling plots respectively in peanut field,persimmon field,orange field and peach field were selected and investigated,the PI and MCN‰ of root-tip cells under soil extracts from all plots were determined. By using micronucleus test technique of Vicia faba root-tip cells,this paper evaluated the pollution status of soil in Pingle Manganese Mine wasteland of Guangxi,and analyzed the reasons that might cause soil pollution. The results showed that the MCN‰ of root-tip cells of V.faba treated with the soils by means of maceration was obviously higher than that of the cells treated with the negative control group(P<0.05); orange field was under the most serious pollution,the PI had reached up to 2.93,while peanut field got the least,the PI was 1.97. In conclusion,these indicated that the soil in this wasteland was more or less polluted,suggesting that it's not suitable for crops at the early stage of farming.