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十种淫羊藿属植物的谷草转氨酶同工酶研究
宁丽丽, 陈庆富
贵州师范大学 生命科学学院 植物遗传育种研究所, 贵阳 550001
摘要:
用聚丙烯酰胺凝胶电泳技术对淫羊藿属植物10个种(含7个大花种和3个小花种)32个幼叶样品的谷草转氨酶同工酶进行了研究。结果发现,谷草转氨酶同工酶酶带共15条。不同物种的酶带数为2~15条,不同植株的谱带数为2~10条。种间存在明显差异,可以作为物种鉴定的辅助依据。
关键词:  淫羊藿属  谷草转氨酶同工酶  系统关系
DOI:10.3969/j.issn.1000-3142.2011.01.022
分类号:Q945
Fund project:
Glutamate oxaloacetate transaminase isozyme in 10 species of genus Epimedium
NING Li-Li, CHEN Qing-Fu*
Institute of Plant Genetics and Breeding, School of Life Science, Guizhou Normal University, Guiyang 550001, China
Abstract:
The glutamate oxaloacetate transaminase isozyme in 32 young leaf samples of 10 species of Epimedium(including 7 species of the big-flower group and 3 species of the small-flower group)were studied by means of polyacrylamide gel eletrophoresis(PAGE). The results showed that 15 bands of glutamate oxaloacetate transaminase isozyme were discovered in all accessions,likewise,2-15 bands in different species and 2-10 in different plant individuals,showing that zymographs among species were obviously different. Therefore,this could be an auxiliary evidence for the species identification.
Key words:  Epimedium  glutamate oxaloacetate transaminase isozyme  phylogeny
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