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红背叶提取物的体外抗氧化活性
鲁俊华1,2, 陈月圆2, 黄荣韶1, 陈换莹3, 李典鹏2, 文永新2*
1.广西大学 农学院, 南宁 530004;2. 广西壮族自治区 中国科学院 广西植物研究所, 广西 桂林 541006;3.广西师范大学, 广西 桂林 541001
摘要:
采用DPPH法、FRAP法、ABTS法和清除羟基自由基法四种抗氧化活性方法,测定了红背叶不同溶剂萃取60%EtOH提取物得到的部位清除自由基的能力。结果表明,乙酸乙酯提取物的抗氧化能力最强,强于阳性对照VC和Trolox; 其次是60%乙醇提取物,其抗氧化能力基本与阳性对照BHA相当。
关键词:  DPPH  FRAP  ABTS  羟基自由基  抗氧化活性  红背叶
DOI:10.3969/j.issn.1000-3142.2011.01.028
分类号:Q946
Fund project:
Study on the antioxidant activity of extracts from the leaves of Alchornea trewioides
LU Jun-Hua1,2, CHEN Yue-Yuan2, HUANG Rong-Shao1, CHEN Huan-Ying3, LI Dian-Peng2, WEN Yong-Xin2*
1.College of Agriculture, Guangxi University, Nanning 530004, China;2.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China;3.Guangxi Normal University, Guilin 541001, China
Abstract:
Antioxidant activity of different solvents extracts fractions fractionated from 60% EtOH extracts of the leaves of Alchornea trewioides were evaluated by DPPH assay,FRAP assay,ABTS assay and scavenging the corresponding hydroxyl radical method. When compared with the positive control samples BHA,VC and Trolox,the determination results showed that antioxidant activity of EtOAc extracts fractions of the leaves of A.trewioides was the highest,its antioxidant activity was higher than VC and Trolox. Antioxidant activity of 60% ethanol extracts fractions was as much as BHA,but lower than VC and Trolox.
Key words:  DPPH  FRAP  ABTS  OH·  antioxidant activity  Alchornea trewioides
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