樟树叶挥发性成分研究

Essential oils from the leaves of Cinnamomum camphora

关键词:  香樟  挥发油  GC/MS  枯叶  老叶  嫩叶  抗氧化

Key words:  Cinnamomum camphora  essential oil  GC/MS  dead leaves  older leaves  young leaves  antioxidant

摘要:

通过GC/MS方法,对湖南樟树嫩叶、老叶以及枯叶挥发油的成分和抗氧化性能进行了研究,分析鉴定了其中含量占95%以上的31个化合物。结果表明:嫩叶中以Copaene(28.55%)、石竹烯(25.81%)和α-石竹烯(12.69%)为主要成分; 老叶挥发油以芳樟醇含量最高(78.30%); 枯叶挥发油主含石竹烯(38.64%)、芳樟醇(19.36%)、L-樟脑(18.69%)和α-石竹烯(17.66%)。三种挥发油均有抗氧化能力,清除ABTS自由基能力大小依次为:老叶>枯叶>嫩叶; 脂质体系清除能力大小依次是:枯叶>老叶>嫩叶。

Abstract:

In this paper,the phytochemical components of the essential oils from young leaves,older leaves and dead leaves of Cinnamomum camphora were examined,which resulted in the identification of 31 compounds representing 95% of the essential oils. Linalool(68.51%),the main components identified in the oil of young leaves were Copaene(28.55%),Caryophyllene(25.81%)and alpha-Caryophyllen(12.69%); the dominant component in the essential oil of older leaves was Linalool(78.30%).Caryophyllene(38.64%),Linalool(19.36%),L-camphor(18.69%)and alpha-Pinene(17.66%)were the major components in the essential oil of the dead flowers. The antioxidant potency of three essential oil from C.camphora were investigated by employing two established in vitro systems,such as ABTS⁺ and Lipid Peroxides(LPO). All the three essential oils significantly showed ability of trapping free radicals,and thereby inhibition of lipid peroxidation. The orders of scavenging activity of different essential oils were,free radicals:older leaves>dead leaves>young leaves; lipid peroxidation:dead leaves>older leaves>young leaves.