| 摘要: |
| 试验以睫毛萼凤仙花无菌苗的叶片、茎和根为外植体进行离体培养,并对两种具有不同再分化能力的愈伤组织进行电镜超微观察。结果表明:在培养基MS+NAA 0.5 mg/L+6-BA 0.5 mg/L上愈伤组织诱导率达最高,叶片愈伤组织的诱导率为100%,茎的愈伤组织诱导率为67%,而根却没有愈伤组织的发生; 叶片愈伤组织在MS+NAA 0.5 mg/L+6-BA 2.0 mg/L上培养40 d后有不定芽的发生,而茎的愈伤组织却没有器官发生; 电镜观察表明,具有再分化能力的愈伤组织的细胞排列不规则,其表面有较明显的类似组织分化。 |
| 关键词: 睫毛萼凤仙花 离体培养 超微观察 |
| DOI：10.3969/j.issn.1000-3142.2011.03.023 |
| 分类号:Q813.1 |
| Fund project: |
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| Tissue culture of Impatiens blepharosepala and ultrastructural observation of callus |
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WANG Xue-Juan1, CHENG Xin-Li2, CHAI Rui-Juan3
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1.College of Urban Construction and Environment, Anhui Science and Technology University, Fengyang 233100, China;2.Landscape development and construction of tianjin co.Ltd, Tianjin 300203, China;3.Department of Bioscience
and Engneering, Anhui Technology and Science College, Wuhu 241000, China
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| Abstract: |
| Leaves,stem and root of Impatiens blepharosepala seedling were cultured in vitro,and two types of callus having different differentiation were observed by electron microscope. The results showed that the induction rate was highest on MS+NAA 0.5 mg/L+6-BA 0.5 mg/L,the induction rate of leaves was 100% and the rate of stems was 67%,but the roots can't be induced. Callus of leaves can be induced buds on MS+NAA 0.5 mg/L+6-BA 2.0 mg/L after 40 d,but there was no organ generated from stems. Cells of callus having differentiation arrayed irregularly and there was obviously similar tissue differentiation by electron microscope. |
| Key words: Impatiens blepharosepala culture in vitro ultrastructural observation |
