珍稀濒危植物银鹊树体细胞胚时期同工酶分析

Studies on isozymes during somatic embryogenesis of Tapiscia sinensis

关键词:  银鹊树  体细胞胚  聚丙烯酰胺凝胶电泳  同工酶  酶带

Key words:  Tapiscia sinensis  somatic embroyos  Polyacrylamide gel electrophoresis  isoenzyme  enzyme bands

摘要:

采用聚丙烯酰胺凝胶电泳技术对银鹊树体细胞胚胎发生过程中的酯酶(EST)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和淀粉酶（AMY）进行了同工酶分析。结果表明：球形胚时期的EST、POD、SOD、AMY同工酶活性最强；在体细胞胚胎形态建成过程中，SOD同工酶有新酶的合成，而POD同工酶则表现为活性表达增加并有新酶合成，AMY同工酶活性也表现为不断增加的趋势。说明在银鹊树体细胞胚胎发生过程中，EST、SOD、POD和AMY同工酶酶谱变化与其体细胞胚的发生和发育过程密切相关，这些同工酶的酶谱变化及活性的强弱可作为其体细胞胚时期生理生化阶段转变的标志。

Abstract:

Polyacrylamide gel electrophoresis was used as a tool to analyze isozyme patterns such as peroxidas〖JP3〗e(POD)，esterase(EST)，amylase(AMY)and superoxide dismutase(SOD)in somatic embryogenesis of Tapiscia sinensis. The results showed that a higher expression of EST，POD，AMY and SOD isoenzymes indicated that there was a higher physiological metabolic rate in the stage of globular embryos，and SOD became more which might be associated with the processes of somatic cells from the differentiation into embryonic regeneration. POD continued to change and strengthen in the process of somatic embryogenesis；At the same time，the expression of AMY also was strengthened. The activity variations of POD，EST，AMY and SOD were closely related to the induction of embryogenic callus and the development of somatic embryos in somatic embryogenesis of T.sinensis. These〖JP2〗 enzymes could be used as the signs during the development of somatic embryos，and would be as a reference label of the specific developmental stages.