姜荷花炭疽病病原菌分离与鉴定

Isolation and identification of pathogens that caused Curcuma alismatifolia anthracnose

关键词:  姜荷花  炭疽病  分离  鉴定

Key words:  Curcuma alismatifolia  anthracnose  isolation  identification

摘要:

利用常规组织分离法对姜荷花炭疽病病原菌进行了分离，并对分离菌株进行培养、纯化、回接和重新分离，最后利用形态学和分子生物学技术对致病菌株进行了鉴定。结果表明：从感病叶片中分离得到6株病原菌，病原菌室外回接发现只有菌株Cum 3致病，Cum 3室外回接致病率96.7%，室内回接致病率100%。形态学鉴定，Cum 3为镰孢酶属病原菌，菌落圆型，菌丝白色，有隔膜，有分枝；分生孢子有小型、大型和厚垣3种。对菌株Cum 3进行ITS序列分析，获得全长528 bp的序列，该序列与Genbank中已登陆的序列进行相似性分析，并结合田间致病症状，认为该致病菌为层出镰孢菌。

Abstract:

The pathogens that caused Curcuma alismatifolia anthracnose were isolated using usual tissue isolation. Strains were cultivated，purified，reinoculated in the field and laboratory. The strain that caused the anthracnose was re isolated. Morphological and molecular biological techniques were used to identify the strain. Results showed that 6 strains were isolated from infected leaves of C.alismatifolia，but only Cum 3 caused the anthracnose. The incidences of Cum 3 disease reinoculated in the field and laboratory were 96.7% and 100% respectively. Cum 3 is a kind of fungi belonging to the genus Fusarium，and its colonial morphology is a circular form，with white mycelium，diaphragms and branches. There are three kinds of conidia:microconidia，macroconidia and chlamydospores. At last，the sequence of internal transcribed spacer (ITS) region of Cum 3 was analyzed，the length was 528bp and the sequence was compared with other species in the Genbank. The symptom in the field indicates that the pathogen of Curcuma alismatifolia anthracnose is Fusarium proliferatum.