姜花属种间杂交胚拯救研究

熊友华1; 2; $22; $21*

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姜花属种间杂交胚拯救研究
熊友华1，2，庄雪影2，刘念1*
1．仲恺农业工程学院园艺园林学院，广州 510225;2.华南农业大学林学院，广州 510642

摘要:

为提高姜花属种间杂交胚挽救中幼胚萌发率，以白姜花×金姜花的胚珠为试材，研究不同胚珠发育时期、不同培养基及低温处理果实对幼胚萌发率的影响。结果表明，白姜花×金姜花胚挽救的适宜培养基是MS+0.1 mg/L BA＋0.1 mg/L NAA；接种时期以60 d的幼胚培养效果最佳；低温处理果实3～6 d能有效提高幼胚的萌发率。

关键词: 姜花属种间杂交胚拯救

DOI：

分类号:Q943.1

Fund project:

Study on embryo rescue of interspecific hybrids between Hedychium

XIONG YouHua1，2， ZHUANG XueYing2， LIU Nian1*

1.College of Horticulture and Landscape Architecture， Zhongkai University of Agriculture and Engineering， Guangzhou 510225， China;2.College of Forestry， South China Agricultural University， Guangzhou 510642， China

Abstract:

The object of this study was to improve the germination capacity of embryoes of Hedychium hybrids through embryo rescue in vitro. Hybrid ovules of H.coronarium×H.gardnerianum were used to investigate the effect of different growth stage of embryo，different culture medium and low temperature treatment on the germination capacity of embryoes. The results indicated that MS medium supplemented with 0.1 mg/L BA and 0.1 mg/L NAA showed suitable for embryo rescue in vitro culture. It was showed that the optimum germination capacity of embryoes reached 40.6%，which was obtained by taking 60 day old immature embryo as explant. Low temperature treatment of fruits for 3-6 d，had a positive effect on improvement of the germination capacity of embryoes.

Key words: Hedychium interspecific hybridization embryo rescue