脱油油樟叶提取物的体外抑菌活性研究

张超1; $21; $21*; $21; $22; $23

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脱油油樟叶提取物的体外抑菌活性研究
张超1，魏琴1，杜永华1*，周黎军1，蒋勤久2，殷中琼3
1.宜宾学院生命科学与食品工程学院发酵资源与应用四川省高校重点实验室，四川宜宾 644000;2.宜宾县生产力促进中心，四川宜宾 644000;3.四川农业大学动物疫病与人类健康四川省重点实验室，四川雅安 625014

摘要:

探讨了油樟叶提取挥发油后的残渣的乙醇浸膏和乙醇浸膏的石油醚萃取相、乙酸乙酯萃取相、正丁醇萃取相和水萃取相对大肠杆菌、金黄色葡萄球菌、沙门氏菌等三种常见致病菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC)以及抑菌曲线。结果显示，各提取物对大肠杆菌的抑菌活性为正丁醇萃取相（MIC、MBC：7813、15.625 mg/mL）＞乙酸乙酯萃取相（MIC、MBC：15.625、31.125）＞乙醇浸膏和水萃取相（MIC、MBC：31.25、62.5）＞石油醚萃取相（MIC和MBC都高于500）；对金黄色葡萄球菌的抑菌活性为石油醚萃取相和乙酸乙酯萃取相（MIC、MBC：7813、15.625）＞正丁醇萃取相（MIC、MBC：15.625、31.125）＞乙醇浸膏和水萃取相（MIC、MBC：3125、62.5）；对沙门氏菌的抑菌活性为乙酸乙酯萃取相（MIC、MBC：3.9063、7.813）＞正丁醇萃取相（MIC、MBC：15.625、31.125）＞乙醇浸膏和水萃取相（MIC、MBC：31.25、62.5）＞石油醚萃取相（MIC、MBC：31.25、125）。由此可见，油樟叶提取物中存在抗菌活性物，其活性成分主要集中在乙酸乙酯萃取相和正丁醇萃取相。曲线结果表明，脱油后的油樟叶提取物对三种细菌的作用均属于浓度依赖型。

关键词: 油樟叶提取物抑菌活性

DOI：

分类号:S644.6，Q93

Fund project:

The antibacterial study on extracts from de oiled leaves of Cinnamomum longepaniculatum against three of the pathogenic bacteria

ZHANG Chao1， WEI Qin1， DU YongHua1*， ZHOU LiJun1， JIANG QinJiu2， YIN ZhongQiong3

1.College of Life Science and Food Engineering Key Lab of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan， Yibin 644000， China;2.Yibin County Productivity Promotion Center， Yibin 644000， China;3.Key Laboratory of Animal Disease and Human Health of Sichuan Province， Sichuan Agricultural University， Ya’an 625014， China

Abstract:

The antimicrobial activity of ethanol extracts from the de oiled leaves of Cinnamomum longepaniculatum and system solvent extraction by petroleum，ethyl acetate and N butanol against the Escherichiu coli，Staphylococcus aureus，Salmonella. Minimum inhibition concentration(MIC)，minimal bacteriocidal concentration（MBC）and time kill curves of extracts were studied. And the activities against the three strains of bacterial were compared. The resultrs showed that，for E.coli，the N butanol extracts（MIC，MBC：7.813，15.625 mg/mL）＞ethyl acetate extracts（MIC，MBC:15.625，31.125）＞the ethanol extracts and water extracts（MIC，MBC:31.25，62.5）＞petroleum extracts（MIC and MBC＞500）. For S.aureus，petroleum extracts and ethyl acetate extracts（MIC，MBC：7.813，15625）＞N butanol extracts（MIC，MBC:15.625，31.125）＞ethanol extracts and water extracts（MIC，MBC:31.25，62.5）. For Salmonella，ethyl acetate extracts（MIC，MBC:3.9063，7.813）＞N butanol extracts（MIC，MBC:15.625，31.125）＞water extracts（MIC，MBC:31.25，62.5）＞petroleum extracts（MIC，MBC:31.25，125）. C.longepaniculatum had some antibacterial active compounds and the main active constituents existed in ethyl acetate extracts and N butanol extracts. The extracts of de oiled leaves of C.longepaniculatum displayed concentration dependent relationship with all the three pathogenic bacteria.

Key words: Cinnamomum longepaniculatum leaves extracts antibacterial activity