采用高效液相色谱技术分析烟草体内的维生素B6化合物

Analysis of vitamin B6 vitamers in tobacco plants by high performance liquid chromatography

关键词:  烟草  高效液相色谱  维生素B6  存在形态

Key words:  tobacco plants  high performance liquid chromatography  vitamin B6  existing forms

摘要:

维生素B6（VB6）是一类化合物的总称。近年来研究发现VB6在植物体内发挥抗逆作用。烟草作为模式植物其体内VB6的存在形态还未见报道。本研究采用高效液相色谱结合荧光检测技术对烟草体内VB6的存在形态进行了分析。结果表明：土壤栽培烟草叶、茎和根中VB6的含量依次为2.9、1.7、3.0 μg/g鲜重；组培烟草叶片的VB6含量为3.9 μg/g鲜重，构成比为磷酸吡哆胺（PMP）7%、吡哆胺（PM）14%、磷酸吡哆醛（PLP）19%、吡哆醛（PL）29%、吡哆醇（PN）30%；组培烟草在连续3周的检测过程中，PLP和PL含量下降、PN含量上升，VB6总量保持相对稳定。研究结果有助于以烟草为材料，进一步开展植物体内VB6代谢机制和特殊生理机制的研究。

Abstract:

Vitamin B6(VB6)is the general term for a kind of chemical compounds .VB6 exists in several forms，and has been linked to stress responses in plants. Until now no reports about the distribution of B6 vitamers in tobacco plants have been observed. In our experiment，the determination of VB6 vitamers in tobacco plants was described by using HPLC with fluorescence detector. The results indicated that，the contents of VB6 in leaves，tender stem and roots were 2.9，1.7 and 3.0 μg/g fresh weight，respectively. Leaves of tobacco plants grown on MS basal media exhibited a high content of 3.9 μg/g fresh weight. The constituent ratio of B6 vitamers were as follows: pyridoxamine 5′ phosphate(PMP) 7%，pyridoxamine(PM) 14%，pyridoxal 5′ phosphate(PLP) 19%，pyridoxal(PL) 29% and pyridoxine(PN) 30%. During the determination period of three weeks，the contents of PLP and PL decreased，and that of PN increased. The amount of VB6 was relatively constant. Our results would be favorable for further study on the metabolic mechanism and special physiological mechanism in tobacco plants.