广西小叶红叶藤挥发油化学成分及抗氧化性研究

Chemical constituents and antioxidant activity of essential oils from Rourea microphylla in Guangxi

关键词:  小叶红叶藤  挥发油  抗氧化性  气相色谱 质谱联用

Key words:  Rourea microphylla  essential oils  antioxidant activity  GC MS.

摘要:

采用气相色谱 质谱联用技术对小叶红叶藤挥发油的化学成分进行分析，并用DPPH•法、ABTS•+法和铁氰化钾还原法对其抗氧化能力进行研究。结果表明:从小叶红叶藤挥发油分离出221个色谱峰，共鉴定64个化合物，占挥发油总量的9572%，主要成分为dl 薄荷酮（5343%）、(E) 薄荷酮（1420%）、5 甲基 2 异丙基 3 环己烯 1 酮（987%）、β 萜品烯（816%）、β 蒎烯（154%）、α 蒎烯（118%）等。抗氧化实验中，不同浓度的挥发油对DPPH和ABTS+自由基有较好的清除能力，最高清除率分别达到6225%和6811%，但其还原能力较弱，抗氧化能力均具有明显的量 效关系。

Abstract:

The constituents of the essential oils of Rourea microphylla from Guangxi were separated and identified by GC MS. The scavenging activities on DPPH• radical，ABTS•+radical and reducing power were detected by UV Vis spectrophotometry. 221 compounds were isolated and 64 compounds were identified that composed about 9572% of the total essential oils. The principal chemical constituents of the essential oils were dl Menthone(5343%)，(E) Menthone(1420%)，2 isopropyl 5 methyl 3 Cyclohexen 1 one(987%)，β Terpinene(816%)，β Pinene(154%)，α Pinene(118%)，et al. These oils possessed good scavenging activities on both DPPH• and ABTS•+ radical，the scavenging rate reaching to 6225% and 6811%，respectively，but showed low activities on reducing power. All antioxidant activity showed a concentration effect relationship.