蛇苔过氧化物酶基因的序列分析与功能预测

Sequence analysis and function predication of poroxidase from Conocephalum conicum

关键词:  过氧化物酶  蛇苔  序列分析  功能预测

Key words:  peroxidases  Conocephalum conicum  sequence analysis  function predication

摘要:

过氧化物酶在生物界分布极广，在细胞代谢的氧化还原过程中起重要的作用。为深入研究蛇苔过氧化物酶的理化性质及功能，对其同源性，亚细胞定位，结构和功能等进行了生物信息学预测和分析。结果表明：该序列有1 050 bp长的开放阅读框，编码349个氨基酸，与拟南芥等的POD相似性较高（相似性为71％）。预测R68、F71、H72、P170、H200和D276为该酶的活性位点，C41与C122、C74与C79、C128与C328、C207与C239形成四对二硫键，对蛋白质的功能、天然构象和稳定性有重要作用，为研究苔类植物过氧化物酶的作用机制和功能等奠定了基础。

Abstract:

Peroxidases is widespread in biological world. It plays an important role in the cell redox metabolism.In order to furher study the characteristics and functions of Conocephalum conicum poroxidase，we performed bioinformatics analysis of its homology，subellular location，tertiary structure，function and so on. The results showed that the sequence had a complete open read frame of about 1 050 bp and encodes 349 amino acids，and the sequence had similar POD with that of Arabidopsis thaliana(the similarity beween them is 71%). This sequence was predicated to be C.conicum poroxidase which belonged to the poroxidase super family，and R68、F71、H72、P170、H200 and D276 to be the C.conicum POD’s active sites in which the four groups of disulfide bonds：C4 and C122、C74 and C79、C128 and C328、C207 and C239 played a very important role in maintaining the protein’s function，natural conformation and stability. This study would set an important foundation for the study of the interaction mechanisms and functions of the C.conicum poroxidase.