茅苍术愈伤组织诱导及其细胞悬浮培养研究

Research on callus induction and cell suspension culture of Atractylodes lancea

关键词:  茅苍术  愈伤组织  细胞悬浮培养

Key words:  Atractylodes lancea  callus  cell suspension culture

摘要:

采用正交试验法研究了不同的植物生长调节剂对茅苍术叶柄、叶片和根茎愈伤组织诱导的影响，结果表明，不同外植体在各自的最佳培养条件下，叶柄、叶片和根茎愈伤组织的诱导率分别为99.0%、83.5%和71.5%，以叶柄的培养效果最好，其中2，4 D对茅苍术愈伤组织的诱导具有极显著的效果，在各种植物生长调节剂组合中，诱导叶柄愈伤组织形成的最佳组合为0.4 mg•L 1NAA、4.0 mg•L 1 2，4 D和0.4 mg•L 1 KT，培养20 d左右，诱导率达到99.0%。此外，将茅苍术叶柄细胞悬浮培养至18 d时，细胞量、多糖和苍术素的含量均达到最大值，分别为9.07 g•L 1、15.68 mg•L 1和19.62 ug•L 1。

Abstract:

Effect of different plant growth regulator on petiole，leaf and rhizome callus induction was studied by orthogonal design in this paper. It showed that petiole，leaf and rhizome callus induction rate were 99.0%、83.5% and 71.5% in their optimum culture conditions respectively. And Petiole was the most easy to form callus. There was a significant promotion effect of 2，4 D on the callus induction. The optimal composition for petiole callus induction was 0.4 mg•L 1NAA、4.0 mg•L 1 2，4 D and 0.4 mg•L 1 KT；and induction rate reached 99.0% when petiole being cultured for 20 d. In addition，when cell from petiole callus was cultured for 18 d，the maximum yields of cell biomass，polysaccharide and atractylodin were 9.07 g•L 1、15.68 mg•L 1 and 19.62 ug•L 1respectively.