水稻无侧根突变体生理生化变化的研究

Physiological and biochemical changes in lateral root mutant of rice

关键词:  水稻  侧根突变体  酶活性  蛋白质电泳

Key words:  rice  lateral root mutation  enzymatic activity  protein electrophoresis

摘要:

对水稻无侧根突变体RM109，原品种大力及杂交后代F1三者的部分生理指标进行了比较。包括抗氧化酶（SOD、POD、CAT）和琥珀酸脱氢酶（SDH）的活性及叶绿素、丙二醛（MDA）、脯氨酸（Pro）等的含量，以及可溶性蛋白质的电泳分析。结果表明：RM109超氧化物歧化酶（SOD）活性为大力的50%，F1为大力的72%；RM109过氧化物酶（POD）活性比大力高125%，F1的比大力低3%；RM109过氧化氢酶（CAT）活性为大力的56%，F1为大力的11%；RM109琥珀酸脱氢酶活性为大力的60%，差异都极显著。揭示了基因突变后引发的生理生化的变化，从中探讨突变机理。

Abstract:

The activities of super oxide dismutase(SOD)，peroxidase(POD)，catalase(CAT)and succinate dehydrogenase(SDH)，the content of chlorophyll，malondialdehyde(MDA)，and proline(Pro)，and the electrophoresis of soluble protein were compared among no lateral root mutant(RM109)，original varieties(Oochikara)and their hybrids(F1). The results showed that SOD activity of Oochikara was stronger than RM109 by 50%，F1 was 72% of Oochikara；the POD activity of RM109 was higher than Oochikara by 125%，F1 was lower than Oochikara by 3%；the CAT activity of RM109 was 56% of Oochikara，F1 was only 11% of Oochikara；the SDH activity of RM109 was 60% of Oochikara. All above differences were up to be very significant. All of the revealed characteristics changes in physiology and biochemics might be caused by the gene mutation.