联合应用ESI MS和1H NMR分析含笑属植物种子磷脂

Application of ESI MS combined with 1H NMR in analyzing seed phospolipids of six species of Michelia

关键词:  种子磷脂  质谱  核磁共振  指纹  含笑属

Key words:  Seed phospholipid  ESI MS  NMR  Fingerprinting  Michelia

摘要:

首次尝试利用电喷雾电离质谱（ESI MS）和氢原子核磁共振（1H NMR）技术分析了含笑属六种植物种子的磷脂特性，发现在两个指纹图谱区发现明显的差异，即在质荷比（m/z）895 910（ESI MS）和5.30～540 mg/L（1H NMR）两个特异的区域存在显著差异。这些源于种子磷脂ESI/MS和1H NMR的谱带差异可以被用来分析不同植物的种子的磷脂特征。而且，相似地，在更广的层面上，特异性的谱带差异可用于分析其他植物种子的磷脂组成和特性，辅助鉴定种子。

Abstract:

To date，there was no example to authenticate seed phospholipids based on their fingerprinting. By means of electrospray ionization mass spectrometry(ESI MS)and proton nuclear magnetic resonance(1H NMR)spectroscopy，phospholipids fraction extracted from seeds of six species from the genus Michelia were detected. It was firstly found that distinct difference in spectral fingerprinting region between m/z 895 910 in ESI MS and 5.30-5.40 mg/L in 1H NMR among these six species. Thus，it suggested that the spectral differences shown by ESI MS and 1H NMR among seeds of these six species could be applied to identify them. And in a wider sense，for analyzing seed phospholipids of other plant species，a combination of ESI MS AND NMR was an effective tool.