韭葱黄条病毒、洋葱黄矮病毒和胡葱黄条病毒六安分离物CP基因克隆及同源性分析

韦传宝1; 2*; $21; $21; $21; $21

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韭葱黄条病毒、洋葱黄矮病毒和胡葱黄条病毒六安分离物CP基因克隆及同源性分析
韦传宝1，2*，华俊雅1，杨宇1，隗洋洋1，史利利1
1.皖西学院生物与制药工程学院，安徽六安 237012;2.安徽省植物生物技术实训中心，安徽六安 237012

摘要:

设计特异性引物PCR扩增了六安大蒜病样中的韭葱黄条病毒（Leek yellow stripe virus，LYSV）、洋葱黄矮病毒（Onion yellow dwarf virus，OYDV）和胡葱黄条病毒（Shallot yellow stripe virus，SYSV）的全长CP基因，插入到pGEM T载体并测序。分别比较3种病毒CP基因种内变异性和种间亲缘关系。结果表明LYSV六安分离物CP基因由864个碱基组成，与Genbank上已报道的68个LYSV不同分离物CP基因的核苷酸序列同源性为76.12%～84.31%；OYDV的CP基因由771个碱基组成，与Genbank上已报道的86个OYDV不同分离物同源性为81.06%～90.40%；SYSV的CP基因由774个碱基组成，与Genbank上已报道的11个SYSV不同分离物CP基因同源性为88.63%～94.32%；从分析结果来看，LYSV的CP基因不同分离物之间变异性较大，OYDV CP变异性不大，SYSV变异性很小；3种病毒都有1个以上的宿主，病毒种内不同宿主分离物之间CP序列差异很小。进化分析显示OYDV和SYSV的CP基因亲缘性较近并成簇，LYSV的CP基因与OYDV和LYSV的CP基因亲缘性较远。

关键词: 韭葱黄条病毒洋葱黄矮病毒胡葱黄条病毒 CP基因序列分析

DOI：

分类号:S436.421

Fund project:

CP gene cloning and sequence analysis of Liu’an isolates of Leek yellow stripe virus， Onion yellow dwarf virus and Shallot yellow stripe virus

WEI ChuanBao1，2*， HUA JunYa1， YANG Yu1， WEI YangYang1， SHI LiLi1

1.College of Biological and Pharmaceutical Engineering， Western Anhui University， Liu’an 237012， China;2.2.Plant Biotechnological Training Center of Anhui Province， Liu’an 237012， China

Abstract:

Specific primers were designed to amplify CP genes of Leek yellow stripe virus(LYSV)，Onion yellow dwarf virus(OYDV)and Shallot yellow stripe virus(SYSV)Liu’an isolates. Then the CP genes were cloned into pGEM T vectors and were sequenced. Sequence analysis indicated that CP genes of LYSV，OYDV and SYSV Liu’an isolates consists of 864，771 and 774 nucleotide acids respectively. Multiple aligments showed that CP gene of LYSV Liu’an isolate shared 76.12%-84.31% nucleotide acids identities with 68 LYSV CP genes reported on Genbank，CP gene of OYDV Liu’an isolate shared 81.06%-90.40% nucleotide acids identities with 86 OYDV CP genes reported on Genbank and CP gene of SYSV Liu’an isolate shared 88.63%-94.32% nucleotide acids identities with 11 SYSV CP genes reported on Genbank. These results indicated that the divergence in LYSV CP among different isolates was high，whilst divergence of OYDV CP was moderate in different isolates. All the results indicated that the variability of LYSV CP gene among different isolates was high，OYDV CP gene among different isolates was in the middle and SYSV CP gene among different isolates was low. Phylogenetic analysis showed that LYSV CP had little relationship with OYDV CP and SYSV CP and OYDV had closer relationship with SYSV than LYSV.

Key words: Leek yellow stripe virus Onion yellow dwarf virus Shallot yellow stripe virus CP gene sequence analysis