马铃薯遗传转化体系的优化及玉米淀粉分支酶基因SBEⅡb的导入

范亚丽1; $22*; $22

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马铃薯遗传转化体系的优化及玉米淀粉分支酶基因SBEⅡb的导入
范亚丽1，阮颖2*，刘春林2
1.阜阳师范学院生命科学学院，安徽阜阳 236037;2.湖南农业大学作物基因工程省重点实验室，长沙 410128

摘要:

以马铃薯脱毒试管苗茎段为转化受体材料，建立并优化了农杆菌介导的马铃薯遗传转化体系。通过农杆菌介导法将玉米淀粉分支酶基因(Starch branching enzyme b，SBEⅡb)的过表达载体转化马铃薯，接种762个茎段，共获得35株抗性植株。经PCR检测获得了4株转基因阳性植株；对转基因植株进一步进行GUS活性组织化学染色，发现转基因植株的茎段与试管薯均被染上蓝色，表明外源SBEⅡb基因已整合到马铃薯基因组，且正常表达。

关键词: 玉米淀粉分支酶基因马铃薯遗传转化淀粉

DOI：

分类号:Q78

Fund project:

Optimization of genetic transformation system of potato and introduction of maize starch branching enzyme gene SBEⅡb into potato

FAN YaLi1， RUAN Ying2*， LIU ChunLin2

1.School of Life Sciences, Fuyang Teachers College， Fuyang 236037， China;2.Crop Gene Engineering Key Laboratory， Hunan Agriculture University， Changsha 410128， China

Abstract:

The highly effective potato transformation systems were established and optimized using tube potato plantlets as transformation materials in this study. The maize starch branching enzyme 〖JP〗gene(SBEⅡb)was transformed into potato using Agrobacterium tumefaciens mediated transformation method. 762 stem sections were infected by Agrobaterium mediated tumefacien method. 4 transgenic plants were obtained by PCR measurement，implying that SBE Ⅱb 〖JP2〗gene had been integrated into potato genome. It was also found that GUS gene could normally be expressed in stem sections and potato microtubers of the trasgenetic potato plants.

Key words: maize starch branching enzyme gene potato genetic transformation starch