拟南芥成花调控LFY基因的选择性剪接

Alternative splicing of flowering regulatory gene LFY in Arabidopsis thaliana

关键词:  LFY基因  成花转变  选择性剪接  差异表达

Key words:  LFY gene  floral transition  alternative splicing(AS)  differential expression

摘要:

为研究拟南芥成花调控基因LFY，我们采用RT PCR方法分离克隆了三种选择性剪接的片段，分别命名为LFY1239，LFY1263和LFY1275。序列分析表明LFY1263包含一个大小为1 263 bp的开放阅读框，与之前报道的LFY基因片段大小相同，而LFY1239在第一外显子的3′端缺失了36 bp，LFY1275在第一内含子的3′末端插入了12 bp。对几种片段表达部位的分析显示，LFY1239只能在营养生长期的莲座叶中表达，而LFY1263和LFY1275在营养生长期和〖JP2〗花期的花器官和莲座叶中都可以检测到，并且，LFY1263呈现出主导地位，LFY1275与LFY1263表达的比例表现为花器官高于莲座叶，该比例的变化可能预示着与成花调控有关。

Abstract:

To study the expression of flowering regulatory gene LFY gene in Arabidopsis thaliana，we used reverse transcription polymerase chain reaction and isolated three alternative splicing(AS)fragments named as LFY1239，LFY1263，and LFY1275 respectively. Sequence analysis confirmed that fragment LFY1263 contained an open reading frame of 1 263 bp，and was identical to the previously reported and predicted fragment，while LFY1239 lacked 36bp at the 3′ end of the first exon，and LFY1275 had an additional 12bp derived from the 3′ end of the first intron. Expression analysis showed that LFY1239 was only detected in rosette during the vegetative stage，while LFY1263 and LFY1275 presented in both floral organs and rosette during both vegetative and flowering stages. Furthermore，LFY1263 appeared to be the most abundant transcript. The expression ratio of LFY1275 to LFY1263 was higher in floral organs than in rosette leaves，which suggested that such ratio might be associated with the flowering regulation.