镧对菹草活性氧清除酶活性及叶细胞超微结构的影响

Effects of lanthanum on the active oxygen scavenging enzyme activities and ultrastructure in Potamogeton crispus leaves

关键词:  镧  菹草  活性氧清除酶  超微结构

Key words:  lanthanum  P.crispus  active oxygen scavenging enzyme  ultrustructure

摘要:

在菹草培养液中施用LaCl3·8 H2O培养菹草，研究了稀土镧对菹草活性氧清除酶活性及叶细胞超微结构的影响。结果表明，菹草在含适宜浓度La3+的培养液中长势均好于对照。较低浓度La3+处理时，菹草叶绿素含量、叶片自发荧光强度、硝酸还原酶（NRase）活性、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性及可溶性蛋白质含量均升高，而超氧化物歧化酶（SOD）活性、细胞膜透性、O 〖ST4.〗2〖HT〗.产生速率及丙二醛（MDA）含量则呈降低趋势，电镜观察结果为La3+对菹草生长的影响提供了细胞学证据。实验认为促进菹草生长的最适La3+浓度为5～10 mg·L 1

Abstract:

The ultrastructural and physiological effects of lanthanum on Potamogeton crispus were studied in present hydroponic experiment. It was found that the foliage grew better in LaCl3·8H2O than in the medium without LaCl3·8H2O. The chlorophyll content，average auto-fluorescent intensity，nitric acid reducase(NRase)activity，peroxidase(POD)activity，catalase(CAT)activity and soluble protein content increased when the plants were treated with lower La3+ concentration，whereas superoxide dismutase(SOD) activity，membrane permeability，O 〖ST4.〗2〖HT〗. generation rate and malondialdehyde(MDA) content decreased. The TEM results provided an ultrastructural evidence for the effect of La3+ on P.crispus. The optimum La3+ concentrations which promote P.crispus growth were suggested to range from 10 to 15 mg·L 1.