中国石竹离体快繁与试管苗玻璃化研究

Study on in vitro propagation and tube plant vitrification of Dianthus chinensis

关键词:  中国石竹  组培快繁  玻璃化

Key words:  Dianthus chinensis  tissue culture and rapid propagation  vitrification

摘要:

研究了培养基组成成分对中国石竹品种Diana F1White组培程序中种子萌发、诱芽增殖、试管苗玻璃化及生根等重要环节的影响。结果表明：（1）种子适宜的萌发培养基为1/2 MS，在此培养基中种子萌发率为83.33%，播种后30 d时无菌苗高度达6.99 cm，叶片数达26.7。（2）在芽诱导阶段玻璃化苗发生率最高可达53.85%。将光照强度提高至2 000 lx后，采用培养基MS+6 BA 3.0 mg/L+NAA 0.3 mg/L+琼脂8.0 g/L+蔗糖8.0 g/L进行诱芽培养，玻璃化苗发生率降至3.33%，外植体出芽率达到90%，单个外植体出芽数达到7.2个。（3）适宜的生根培养基为1/2 MS+1.0 mg/L IBA+琼脂8 g/L+蔗糖40 g/L，培养30 d时生根率为100%，平均根条数达到24.7条，平均根长度达到4.7 cm。试管苗在消毒后的腐殖土中移栽，成活率达95%。该研究结果为Diana F1试管苗的快速繁殖提供科学依据。

Abstract:

Effects of medium ingredients on seeds germination，buds inducement proliferation，tube plant vitrification and rooting were studied in order to establish efficient rapid propagation technology system of Dianthus chinensis cultivar Diana F1 White. The results showed that proper seeds germination medium was 1/2 MS，and seed germination ratio was 83.33%. Height of aseptic tube plant reached to 6.99 cm，and leaf number per plant was 26.7 on 30th day after sowing in medium. Max.vitrification plant ratio was 83.33% during bud inducement period for tube plant of Diana F1 White. The optimal medium for buds inducement was MS+6 BA 3.0 mg/L+NAA 0.3 mg/L+agar 8.0 g/L+sugar 8.0 g/L after light intensity was promoted to 2 000 lx. Bud inducement ratio and buds number per explant were 90% and 7.2 respectively，and the ratio of vitrification tube plants decreased to 3.33% after 30 days’culture. The optimal rooting medium was 1/2 MS+1.0 mg/L IBA+agar 8 g/L+sugar 40 g/L，and rooting percent，roots number and average root length was 100%，24.7 and 4.7 cm respectively after 30 days’ culture. Survival rate of tube plant was more than 95% after they were transplanted in disinfected humus. The above results would provide scientific base for micro propagation of D.chinensis cultivar Diana F1 White.