不同龙眼资源遗传多样性的SCoT和ISSR 比较分析

陈虎1; $21; 2*; $21; $21; $21; $23

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不同龙眼资源遗传多样性的SCoT和ISSR 比较分析
陈虎1，何新华1，2*，黄桂香1，李峰1，姜建初1，朱建华3
1.广西大学农学院，南宁 530004;2.广西作物遗传改良重点实验室，南宁 530007;3.广西农业科学院园艺研究所，南宁 530007

摘要:

应用SCoT和ISSR标记对 36份龙眼资源和1份近缘种龙荔的遗传多样性进行分析。结果表明：12对SCoT引物共扩增出127条带，平均每条引物扩增10.58条带；15条ISSR引物共扩增出117个条带，平均扩增7.8条带。UPGMA聚类结果表明：SCoT标记和ISSR标记分别在相似系数0.672和0.685水平上，均可将37份材料分成6大类群，SCoT和ISSR标记均适用于龙眼材料的遗传多样性分析，如果将两种标记的数据进行综合分析，可以缩小单一标记的误差。研究结果为龙眼种质资源的保存和利用提供了重要的依据。

关键词: 龙眼 SCoT ISSR 遗传多样性

DOI：

分类号:S667.2

Fund project:

Comparison and analysis of SCoT and ISSR markers for genetic diversity of longan

CHEN Hu1， HE XinHua1，2*， HUANG GuiXiang1， LI Feng1， JIANG JianChu1， ZHU JianHua3

1.College of Agriculture， Guangxi University， Nanning 530004， China;2.Key Laboratory of Crop Genetic Improvement in Guangxi， Nanning 530007， China;3.Institute of Horticulture， Guangxi Academy of Agricultural Sciences， Nanning 530007， China

Abstract:

The diversity and genetic relationship among and within thirty six varieties of longan and one Dimocarpus confinis from China，Vietnam and Thailand were analyzed using SCoT（Start Codon Targeted） and ISSR(Inter Simple Sequence Repeat) markers. The results showed 127 strips were amplified by 12 SCoT primers while 117 strips by 15 ISSR primers. The average strips amplified by SCoT and ISSR primers were 10.58 and 7.8，respectively. The genetic relationships were analyzed using unweighted pair group method of arithmetic average cluster analysis(UPGMA)and the genetic Jaccard similarity coefficients were calculated. The cluster analysis results showed that the thirty seven materials could be clustered into 6 groups at the similarity coefficient level of 0.672 and 0.685. Comparison and mixture analysis of the two molecular markers demonstrated that both SCoT and ISSR were efficient approaches for genetic diversity analysis of longan germplasm. These results would provide theoretical basis for longan germplasm storage and utilization in future.

Key words: longan SCoT ISSR genetic diversity