基于SNP分子标记的凹叶木兰遗传多样性初步研究

Preliminary study on genetic diversity of Magnolia sargentiana (Magnoliaceae) through SNP marker

关键词:  木兰属  遗传多样性  植物保护  PCR  SNP标记

Key words:  Magnolia  genetic diversity  plant conservation  PCR  SNP marker

摘要:

凹叶木兰是我国特有的木兰属植物，仅分布于我国四川和云南两省相邻地区，目前已被列入中国物种红皮书名录，属易危物种。该研究以采自四川南部麻咪泽和美姑大风顶两个自然保护区的野生凹叶木兰为材料，利用以PCR和测序为基础的SNP分子标记方法，初步研究了凹叶木兰的遗传多样性。结果表明：在扩增出的4条510 bp长的序列上，平均在73 bp左右的序列长度上能够检测到一个SNP位点，说明两个自然保护区内不同居群的凹叶木兰具有较高遗传多样性；序列间相似度达97%，与引物所对原序列相似度达36%，表明该序列适宜进行凹叶木兰遗传多样性研究。研究结果为进一步开展凹叶木兰遗传多样性的研究及其保护政策的制定提供了参考。

Abstract:

Magnolia sargentiana is a species endemic to China and is listed on the Red List of vulnerable species. It is only found in limited localities in Sichuan and Yunnan provinces. Using samples collected from the Mamize Provincial Nature Reserve and the Meigu Dafengding National Nature Reserve in the southern Sichuan Province，its genetic diversity was investigated with single nucleotide polymorphism (SNP) marker，based on PCR and sequencing technique. In the four sequences (510 bp lengths) that amplified with specific primers，a SNP locus was found on every 73 bp sequence on average，which suggested that the different M.sargentiana populations in the two nature reserves had a high level of genetic diversity；the similarity among the amplified sequences reached to 97%，and 36% of the amplified sequences was similar to the sequence used for making primers，which indicated that the sequence we selected was suitable for genetic research of M.sargentiana. The results would provide useful information for further conservation research and protection policy making on M.sargentiana or other protected species.