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| 过量表达水杨酸羟基酶NahG降低烟草对青枯菌的抗性 |
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党峰峰1, 林金辉1, 陈成聪1, 陈永萍3, 官德义2, 何水林1,2*
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1.福建农林大学 生命科学学院, 福州 35002;2.福建农林大学 作物遗传改良和综合利用
教育部重点实验室, 福州 35002;3.福建农林大学 园艺学院, 福州 35002
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| 摘要: |
| 研究构建了pMDC32-NahG植物表达载体,并获得了其超表达的转基因烟草T1代株系。结果表明:这些株系与其野生型植株没有明显表型差异,但却表现出较野生型植株更敏感的青枯菌侵染抗性。同时,研究还发现NahG的超表达还显著提高了茉莉酸(JA)-依赖的基因NtPR1-b的表达,降低了(SA)-依赖的相关基因NtPR3和NtPRQ的表达。这表明SA累积的缺陷降低了烟草对青枯菌侵染的抗性。 |
| 关键词: 水杨酸 NahG 青枯菌 |
| DOI：10.3969/j.issn.1000-3142.2013.02.007 |
| 分类号:Q943.2 |
| Fund project: |
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| Overexpression of salicylate hydroxylase NahG declines resistance to Ralstonia solanacearum infection in tobacco |
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DANG Feng-Feng1, LIN Jin-Hui1, CHEN Cheng-Cong1,
CHEN Yong-Ping3, GUAN De-Yi2, HE Shui-Lin1,2*
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1.College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.National Education Minster
Key laboratory of Crop Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University,
Fuzhou 350002, China;3.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
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| Abstract: |
| Studies showed that the plant expression vector of pMDC32-NahG was construed in tobacco K326,and obtained NahG-OE T1 lines. Comparing to wild type K326,NahG-OE lines did not exhibited any phenotypic difference,but exhibited higher susceptibility to Ralstonia solanacearum inoculation. Meanwhile,it was found to be coupled with up-regulated expression of JA-depended NtPR1-b,and down-regulated expression of SA-depended NtPR3 and NtPRQ. Results suggested that SA-degrading NahG tobacco plants declined resistance to R.solanacearum infection |
| Key words: salicylic acid NahG Ralstonia solanacearum |
