长叶皇冠的组织培养与快速繁殖技术研究

Studies on tissue culture and rapid propagation technique of Echinodorus subalatus

关键词:  长叶皇冠  短缩茎  组织培养  间歇灭菌

Key words:  Echinodorus subalatus  unelongated stem  tissue culture  intermittent sterilization

摘要:

以长叶皇冠短缩茎为外植体进行离体培养,对外植体灭菌方法以及培养基中不同种类和浓度生长调节剂对增殖、生根的影响等进行研究。结果表明:以0.1%的HgCl₂为灭菌剂,采用8 min+(6～8)min、间歇时间为24 h的间歇灭菌法,可获得20%以上的成活无菌外植体; 试管苗茎纵切成4份后,接种在1/2MS+6-BA 6.0 mg·L^-1增殖培养基上培养42 d,增殖系数可达9.3; 在1/2MS+NAA 0.2 mg·L^-1培养基上培养28 d后,生根率达100%,平均每株约生根18条; 炼苗后移植成活率100%。

Abstract:

The in vitro culture of Echinodorus subalatus was established by using the unelongated stem as explants. The method of surface sterilization of explants,the effects of different plant growth regulators on the multiplication and rooting were investigated. The results showed that 20% sterile explants could be obtained by twice surface treatment with 0.1% HgCl₂ for 8 min and 6-8 min with an interval of 24 h. When cultured on a 1/2 MS multiplication medium supplemented with 6.0 mg·L^-1 of 6-BA for 42 d,the test-tube seedling exhibited a multiplication coefficient of 9.3. For optimal rooting,induction on a 1/2 MS rooting medium supplemented with 0.2 mg·L^-1 of NAA for 28 d could produce rooted plantlets with 100% rooting rate,and each plantlet generated about 18 roots on average. Rooted plantlets were acclimatized and transferred into outdoor aquatic environment with 100% survival rate.