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| 广藿香八氢番茄红素合成酶PcPSY1基因克隆和序列分析 |
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欧阳蒲月1, 刘永亮2, 梁永枢1, 曾少华3, 莫小路4*
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1. 广东食品药品职业学院, 广州 510520;2. 中国科学院 武汉植物园, 武汉 430074;3. 中国科学院 华南植物园, 广州 510650;4. 广东省中药研究所, 广州 510520
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| 摘要: |
| 根据已获得的广藿香转录组数据中的PSY转录本序列,利用Primer 3在线设计基因全长扩增引物,采用RT-PCR方法获得广藿香的八氢番茄红素合成酶(phytoene synthase,PSY)基因。并利用在线分析平台和生物软件对该其因进行生物信息学分析。获得的广藿香PSY1基因长1 550 bp,编码439个氨基酸,命名为PcPSY1,GenBank登录号为KC862310; 预测了PcPSY1 编码蛋白的结构与功能,且基于PSY基因利用NJ法构建了与21个不同物种间的进化树,进化树表明广藿香PcPSY1基因与桂花的PSY序列亲缘关系最近。成功克隆并分析广藿香PcPSY1基因的全长序列,为进一步阐明广藿香萜类代谢途径奠定基础。 |
| 关键词: 广藿香 八氢番茄红素合成酶 PcPSY1基因 基因克隆 生物信息学分析 |
| DOI：10.3969/j.issn.1000-3142.2013.04.004 |
| 分类号:R931 |
| Fund project: |
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| Cloning and sequential analysis of phytoene synthase (PcPSY1)gene in Pogostmen cablin |
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OUYANG Pu-Yue1, LIU Yong-Liang2, LIANG Yong-Shu1,
ZENG Shao-Hua3, MO Xiao-Lu4*
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1. Guangdong Food and Drug Vocational College, Guangzhou 510520, China;2. Wuhan Botanical Garden, Chinese Academy
of Sciences, Wuhan 430074, China;3. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650,
China;4. Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou 510520, China
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| Abstract: |
| Phytoene synthase(PSY)is a key enzyme in plant terpenoid biosynthetic pathway. According the transcriptome dataset of Pogostmen cablin, one unique sequence encoding PSY was discovered. The primers were designed according to the transcript sequence of PcPSY1 from the P. cablin transcriptome dataset with Primer 3 online. The open reading frame of PcPSY1 was cloned using RT-PCR strategy. The cDNA named as PcPSY1 contains a 1 550 bp open reading frame and encodes a predicted protein of 439 amino acids. The GenBank accession number for this gene is KC862310. PcPSY1 has no signal peptide. PcPSY1 is highly homologous to other PSY protein in 21 different species and is more related to that from Osmanthus fragrans. This study cloned and analyzed mechanism of terpenoid biosynthesis in Pogostemon cablin plants. |
| Key words: Pogostemon cablin phytoene synthase PcPSY1 gene cloning bioinformatical analysis |
