| 摘要: |
| 为获得烟草合子胚中的优势表达基因,利用CAP3程序对来自烟草公共数据库的EST序列进行组装,利用MISA程序从组装后的EST中筛选SSR位点,将多态性的SSR位点在烟草合子文库中进行扩增并对等位基因进行分析。结果表明:具有多态性的16个SSR标记中,有9个基因能从烟草的合子库中成功扩增得到。该研究为筛选烟草合子胚中优势表达基因提供新的途径。 |
| 关键词: EST SSR 优势表达基因 合子 烟草 |
| DOI：DOI:10.3969/j.issn.1000-3142.2013.04.005 |
| 分类号:Q948 |
| Fund project: |
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| Polymorphic microsatellite markers from expressed sequences tag in Nicotiana tabacum for obtaining the genes of preponderant expression in zygotes |
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ZHANG Jun-E1, LI Fen2, SUN Meng-Xiang3
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1. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China;2. College of Life Sciences,
Henan Normal University, Xinxiang 453007, China;3. Key Laboratory of Ministry of Education for
Plant Development Biology,College of Life Sciences, Wuhan University, Wuhan 430072, China
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| Abstract: |
| In order to obtain the genes of preponderant expression in zygote cells of tobacco,CAP3 program was used to assemble ESTs from GenBank,and MISA program were used to screen microsatellites. Then polymorphic microsatellites were amplified in cDNA of tobacco zygotes library. The results showed that sixteen polymorphic SSR markers from tobacco ESTs deposited in a public sequence database were developed. Of these,nine genes could be successfully amplified in cDNA of tobacco zygotes library. The study would provide new channel to screen genes of preponderant expression in tobacco zygotes. |
| Key words: EST(expressed sequence tag) SSR(simple sequence repeats) genes of preponderant expression zygotes tobacco |
