培养基成分和培养时间对匍匐翦股颖植株再生的影响

Effects of medium components and subculture period on plantlet regeneration of creeping bentgrass

关键词:  匍匐翦股颖  愈伤组织  2,4-D  6-BA  继代培养

Key words:  creeping bentgrass  callus  2,4-D  6-BA  subculture

摘要:

以匍匐翦股颖成熟种子为外植体,研究了培养基2,4-D浓度、2,4-D和6-BA组合配比、蔗糖浓度对匍匐翦股颖愈伤组织诱导的影响以及愈伤组织再生过程中继代时间、6-BA浓度、蔗糖浓度对愈伤组织分化的影响。结果表明:在MS培养基上,2 mg·L^-1 2,4-D和0.1 mg·L^-1 6-BA的组合最利于愈伤组织的诱导,诱导率高达94%。蔗糖浓度为30 g·L^-1时愈伤组织诱导率最高,为82%; 在再生过程中,当6-BA浓度为1 mg·L^-1时分化率最高(62%),蔗糖浓度为40 g·L^-1时,愈伤组织分化率最高(52%)。经过2次继代培养的愈伤组织(外植体放到培养基后40天)的分化率为最高(71%),随着继代次数增多,分化率逐渐降低,在经过5次继代后(培养100 d)分化率仅有18%。

Abstract:

Mature seeds of creeping bentgrass were used as explants to study the effects of 2,4-D concentration,combination of 2,4-D and 6-BA,and sucrose concentration on callus induction and the effects of subculture time,6-BA concentration,sucrose concentration on plantlet regeneration. The results showed that combination of 2 mg·L^-1 2,4-D and 0.1 mg·L^-1 6-BA produced the best result with 94% callus induction rate,and 30 g·L^-1 sucrose gave the best result,callus induction rate being 82%. During regeneration process,1 mg·L^-16-BA could bring the best differentiation frequency(62%)and 40 g·L^-1 sucrose resulted in the highest plantlet regeneration frequency(52%).The callus after the second time subculture(40 d after explants were laid on medium)gave the highest differentiation frequency(71%)which began to decline as subculture prolonged. After the fifth time subculture(100 d),only 18% differentiation frequency was obtained.