吉粳88高效再生体系的建立

Establishment of an efficient regeneration system of Jijing88

关键词:  吉粳88  培养基  诱导率  分化率  成苗率

Key words:  Jijing 88  culture medium  induction rate  differentiation rate  regeneration rate

摘要:

以吉粳88为材料,通过对不同培养基和激素的筛选,确定最佳的再生体系。结果表明:NMB培养基为吉粳88最适的愈伤组织诱导培养基。通过L₉(3³)正交实验研究最适激素浓度配比,筛选出吉粳88愈伤组织诱导的最佳激素配比为2.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.10 mg/L NAA。在添加2.0 mg/L 6-BA+0.5 mg/L NAA的NMB基本培养基条件下,吉粳88的分化率最高,成培苗率也最高。

Abstract:

Jijing 88 was used as the research material and different media and hormone combinations were selected,in order to obtain its optimal in-vitro regeneration system. The results showed that NMB medium was the most suitable medium for rice callus induction among the 5 medium. The most suitable plant hormone ratio was investigated by using orthogonal test L₉(3³),and the induction rate was the highest when 2.0 mg/L 2,4-D,0.2 mg/L 6-BA,0.10 mg/L NAA were added. The differentiation rate and regeneration rate were the highest when 2.0 mg/L6-BA,0.5 mg/L NAA were added.