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| 8-甲氧基二氢血根碱化感潜能的评估 |
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丁 兰, 张 丽, 李 静, 杨 玲, 令利军, 杨 宁, 刘国安
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1. 西北师范大学 生命科学学院, 兰州 730070;2. 武威市第八中学, 甘肃 武威 73300
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| 摘要: |
| 以莴苣幼苗为材料,检测了分离自细果角茴香的生物碱对莴苣幼苗根生长及根毛发育的影响。结果表明:50～200 μmol·L-1浓度范围内,8-甲氧基二氢血根碱对莴苣幼苗根长显著抑制; 10、20和30 μmol·L-1 8-甲氧基二氢血根碱对莴苣幼苗根毛的长度和数量有显著的抑制作用,且抑制作用均表现了浓度依赖性。通过对莴苣根尖细胞有丝分裂研究发现,50 μmol·L-1的8-甲氧基二氢血根碱能显著抑制根尖细胞的有丝分裂,而且莴苣幼苗净增值率与根尖细胞的有丝分裂之间呈正相关,证明8-甲氧基二氢血根碱主要通过抑制根尖细胞有丝分裂对莴苣根的生长产生影响。 |
| 关键词: 8-甲氧基二氢血根碱 莴苣 生物碱 化感作用 |
| DOI：10.3969/j.issn.1000-3142.2014.01.016 |
| 分类号:Q945.7 |
| Fund project: |
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| Allelopathic potential of 8-methoxy dihydrosanguinarine |
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DING Lan1*, ZHANG Li1, LI Jing2, YANG Ling1,
LING Li-Jun1, YANG Ning1, LIU Guo-An1
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1. College of Life Sciences, Northwest Normal University, Lanzhou 730070, China;2. Wuwei No. 8 Senior High School, Wuwei 733000, China
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| Abstract: |
| Effects of 8-methoxy dihydrosanguinarine isolated from Hypecoum leptocapum on the root growth and root hair development of Lactuca sativa seedlings were studied. The results showed that the root growth was significantly inhibited by 8-methoxy dihydrosanguinarine at the concentration of 50-200 μmol·L-1; the inhibitory effect on root hairs increased with concentration ranging from 10 to 30 μmol·L-1. The inhibitory effects of tested compound on the length and number of root hairs showed a dose-dependent manner. Moreover,the mitosis of apical cells could be inhibited by 8-methoxy dihydrosanguinarine at the concentrations above 50 μmol·L-1 and revealed a positive linear regression with root NGR. Thus,the effect of 8-methoxy dihydrosanguinarine on Lactuca sativa seedlings mainly explored on the inhibition of the mitotic activity. |
| Key words: 8-methoxy dihydrosanguinarine Lactuca sativa alkaloids allelopathy |
