| 摘要: |
| SD大鼠自由饮用绞股蓝汁(绞股蓝汁每天新鲜配制,浓度为每100 g水2 g茶叶,100 ℃的水温浸泡30 min,取上清液),连续给药60 d,取出肝脏,用差速离心法制备肝脏胞浆液及肝脏微粒体,采用双光束紫外分光光度法测定CYP3A、CYP2E1、NADPH-细胞色素C还原酶、UGT、GST的活性及细胞色素b5的含量,结果显示绞股蓝可显著升高细胞色素b5的含量,显著诱导CYP3A、UGT、GST、NADPH-细胞色素C还原酶的活性,但对CYP2E1没有影响。提示绞股蓝与药物合用时,在体内可能会发生代谢性药物相互作用。 |
| 关键词: 绞股蓝 细胞色素P450 谷胱甘肽-S-转移酶 尿苷二磷酸-葡萄糖醛酸基转移酶 大鼠 |
| DOI：10.3969/j.issn.1000-3142.2014.02.013 |
| 分类号:R285.5 |
| Fund project: |
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| Effects of Gynostemma pentaphyllum on the cytochrome P450 and GST, UGT activities in liver of rat |
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ZHUO Shen1, QIN Hai-Guang1, CHEN Jun2, YANG Zi-Ming3*
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1. Guangxi University of Science and Technology, Liuzhou 545005, China;2. Hunan University of Chinese
Medicine, Changsha 410208, China;3. Guangxi Key Laboratory of Functional Phytochemicals
Research and Utilization, Guangxi Institute of Botany, Guilin 541006, China
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| Abstract: |
| The SD rats were maintained on freshly prepared aqueous extract of Gynostemma pentaphyllum(2%)while the control rats were given distilled water for 60 days. Rat liver was taken and liver microsome and cytochyma enzymes was obtained by different velocity centrifugation. Then the total contents and activities of CYP450 and GST,UGT of rats determined by double wavelength ultraviolet spectrophotometry. The results showed that the G. pentaphyllum could increase the content of b5; Increase the activity of CYP3A,UGT,GST,NADPH-cytochtome C reductase; But had no effect on CYP2E1. The study showed that co-administrated with those drugs metabolized by the above major metabolizing enzymes in liver,metabolic herb-dtug interactions would happened. |
| Key words: Gynostemma pentaphyllum CYP450 GST UGT rat |
