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| 热带假丝酵母(Candida tropiclis)去除蔗渣木聚糖酶解副产物的研究 |
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陈海珊1, 石国良2, 覃香香1, 周玉恒1, 蔡爱华1
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1. 广西植物功能物质研究与利用重点实验室, 广西植物研究所, 广西 桂林 541006;2. 唐传生物科技(厦门)有限公司, 福建 厦门 361026
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| 摘要: |
| 该文研究了木糖、木糖醇对木聚糖酶Shearzyme 500 L酶解蔗渣木聚糖的影响。通过热带假丝酵母(Candida tropiclis)转化酶解副产物木糖,解除木糖对木聚糖酶的抑制作用,从而获得高木二糖含量的低聚木糖。结果表明:木糖是Shearzyme 500 L的酶活性抑制物,其抑制作用与溶液中的木糖量成正比; 木糖醇对木聚糖酶无抑制作用; 热带假丝酵母可将蔗渣木聚糖酶解液中的木糖转化为木糖醇而不利用低聚木糖,木二糖占总糖比例由53.09%升高到62.92%,经二次酶解后,木二糖比例可达78.90%。 |
| 关键词: 热带假丝酵母 蔗渣木聚糖 木聚糖酶 木糖 木糖醇 |
| DOI：10.3969/j.issn.1000-3142.2014.03.019 |
| 分类号:Q946 |
| Fund project: |
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| Study onenzymolysis of bagasse xylan by-products of Candida tropiclis |
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CHEN Hai-Shan1, SHI Guo-Liang2, QIN Xiang-Xiang1,
ZHOU Yü-Heng1, CAI Ai-Hua1
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1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of
Botany, Guilin 541006, China;2. Thomson Biotech(Xiamen)PTE Ltd., Xiamen 361026, China
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| Abstract: |
| The effects of xylose,xylitol on enzymolysis of bagasse xylan by xylanase Shearzyme 500 L were studied. Xylooligosaccharide with high xylobiose content was obtained through the enzymolysis of by-product xylose by Candida tropiclis and relieving the inhibitory effect of xylitol on xylanse. The results were as follows: xylose was the inhibitor of Shearzyme 500 L,and its inhibitory effect was in proportion to xylose content in solution; xylitol had no inhibitory effect on xylanase; C. tropiclis could transform the xylose in the solution of bagasse xylan into xylitol without xylooligosaccharide,and xylobiose increased from 53.09% to 62.92% in total sugar,and reached 78.90% through the second hydrolysis |
| Key words: Candida tropiclis bagasse xylan xylanase xylose xylitol |
