野艾蒿挥发油对HeLa癌细胞形态与结构的影响

Effects of essential oil from Artemisia lavandulaefolia on morphology and structure of HeLa cells in vitro

关键词:  野艾蒿  挥发油  细胞形态  细胞结构  HeLa癌细胞

Key words:  Artemisia lavandulaefolia  essential oil  cell morphology  cell structure  HeLa cells

摘要:

采用MTT法检测细胞活力,用倒置显微镜、荧光显微镜和扫描电子显微镜观察细胞形态与结构的变化,用激光共聚焦显微镜观察细胞微管的分布,从而研究了野艾蒿挥发油对HeLa人宫颈癌细胞形态与结构的影响。结果表明:(1)野艾蒿挥发油对HeLa癌细胞的增殖有明显的抑制作用,呈剂量和时间依赖性。(2)野艾蒿挥发油处理HeLa癌细胞24 h后,100、200 μg/mL实验组细胞体积缩小,核染色质凝集、微绒毛消失、细胞表面有泡状突起,微管解聚,呈现典型的凋亡特征; 400 μg/mL实验组细胞膜破裂、胞浆内含物外泄,呈明显的坏死特征。(3)野艾蒿挥发油具有抑制HeLa癌细胞增殖的作用,低、中浓度的野艾蒿挥发油诱导细胞凋亡,而高浓度的野艾蒿挥发油引起细胞坏死。

Abstract:

The effects of essential oil from Artemisia lavandulaefolia on morphology and structure of HeLa cells were investigated. Cell viability was assayed using MTT method. The differences of cell morphology and structure were observed by inverted microscopy,fluorescence and scanning electron microscope. Furthermore,the distribution of microtubules was observed by confocal microscopy. The results showed that essential oil from Artemisia lavandulaefolia could inhibit the proliferation of HeLa cells in dose-and time-dependent manner. Treated with essential oil of Artemisia lavadulaefolia for 24 h,cells in 100 and 200 μg·mL^-1 experiment groups exhibited the typical morphology changes of undergoing apoptosis,such as cell shrinkage,nucleus chromatin condensed,microvilli disappear,bubbly uplift and the microtubules depolymerized. However,the cells in the 400 μg·mL^-1 group showed the necrotic morphology changes including cytomembrane rupture and cytoplasm spillover. Essential oil from Artemisia lavadulaefolia could inhibit the proliferation of HeLa cells in vitro. And low and middle concentrations of essential oil from Artemisia lavadulaefolia could induce apoptosis,whereas high concentration of the compounds resulted in necrosis in HeLa cells.